Supra-and sub-threshold intracellular-like recording of 2D and 3D neuronal networks using nanopillar electrode arrays  

作　　者：Shivani Shukla Joshua L.Schwartz Callum Walsh Wen Mai Wong Vrund Patel Yu-Peng Hsieh Chichi Onwuasoanya Shaoming Chen Andreas Offenhäusser Gert Cauwenberghs Francesca Santoro Alysson R.Muotri Gene W.Yeo Sreekanth H.Chalasani Zeinab Jahed 

机构地区：[1]Shu Chien-Gene Lay Department of Bioengineering,University of California San Diego,La Jolla,CA 92093,USA [2]Aiiso Yufeng Li Family Department of Chemical and Nano Engineering,University of California San Diego,La Jolla,CA 92093,USA [3]Department of Cellular and Molecular Medicine,University of California San Diego,La Jolla,CA 92093,USA [4]Institute for Genomic Medicine,University of California San Diego,La Jolla,CA 92093,USA [5]Molecular Neurobiology Laboratory,The Salk Institute for Biological Studies,La Jolla,CA 92037,USA [6]Institute of Biological Information Processing—Bioelectronics,IBI-3,Forschungszentrum Jülich,Jülich 52428,Germany [7]Neuroelectronic Interfaces,Faculty of Electrical Engineering and IT,RWTH Aachen,Aachen 52074,Germany [8]Department of Pediatrics,University of California San Diego,San Diego,CA,USA [9]Center for Academic Research and Training in Anthropogeny(CARTA)and Archealization(ArchC),University of California San Diego,La Jolla,CA 92093,USA [10]Sanford Stem Cell Education and Integrated Space Stem Cell Orbital Research(ISSCOR)Center University of California San Diego,La Jolla,CA 92093,USA [11]Sanford Stem Cell Institute Innovation Center,University of California San Diego,La Jolla,CA 92093,USA [12]Center for RNA Technologies and Therapeutics,University of California San Diego,La Jolla,CA 92093,USA

出　　处：《Microsystems & Nanoengineering》2024年第6期351-361,共11页微系统与纳米工程(英文)

基　　金：supported by the Air Force Office of Scientific Research YIP award(AFOSR FA9550-23-1-0090);UC San Diego Materials Research Science and Engineering Center(UC San Diego MRSEC),supported by the National Science Foundation(Grant DMR-2011924)to Z.J;performed at the San Diego Nanotechnology Infrastructure(SDNI)of UCSD,a member of the National Nanotechnology Coordinated Infrastructure,which was supported by the National Science Foundation(Grant ECCS-2025752);supported by NIH grants MH107367 and MH131907 to G.W.Y;Runyon Cancer Research Foundation Fellowship to WM;the Hewitt Foundation fellowship to CW;R01NS115591 and Mathers Foundation grants to SC;National Science Foundation CCF-2208771 to GC.

摘　　要：The brain integrates activity across networks of interconnected neurons to generate behavioral outputs.Several physiological and imaging-based approaches have been previously used to monitor responses of individual neurons.While these techniques can identify cellular responses greater than the neuron’s action potential threshold,less is known about the events that are smaller than this threshold or are localized to subcellular compartments.Here we use NEAs to obtain temporary intracellular access to neurons allowing us to record information-rich data that indicates action potentials,and sub-threshold electrical activity.We demonstrate these recordings from primary hippocampal neurons,induced pluripotent stem cell-derived(iPSC)neurons,and iPSC-derived brain organoids.Moreover,our results show that our arrays can record activity from subcellular compartments of the neuron.We suggest that these data might enable us to correlate activity changes in individual neurons with network behavior,a key goal of systems neuroscience.

关 键 词：RECORDING networks RECORD 

分 类 号：R74[医药卫生—神经病学与精神病学]