Power-free plasma separation based on negative magnetophoresis for rapid biochemical analysis  

作　　者：Lin Zeng Chao Liu Yi Yang Shi Hu Ruihan Li Xiaotian Tan Jienan Shen Yi Zhang Shaohui Huang Hui Yang 

机构地区：[1]Research Center for Bionic Sensing and Intelligence,Institute of Biomedical and Health Engineering,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,518055 Shenzhen,China [2]Marine Engineering College,Dalian Maritime University,116026 Dalian,China [3]Research Center for Medical AI,Institute of Biomedical and Health Engineering,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,518055 Shenzhen,China [4]School of Biosciences,University of Chinese Academy of Sciences,101408 Beijing,China

出　　处：《Microsystems & Nanoengineering》2024年第6期435-447,共13页微系统与纳米工程(英文)

基　　金：supported by National Natural Science Foundation of China(52405634,62074155,82227806);National Key Research and Development Program Grant(2023YFF0721500);Guangdong Basic and Applied Basic Research Foundation(2024A1515012320);Shenzhen Science and Technology Innovation Commission(JSGGZD20220822095200001).

摘　　要：We present a versatile platform for label-free magnetic separation of plasma,tailored to accommodate diverse environments.This innovative device utilizes an advanced long-short alternating double Halbach magnetic array,specifically engineered for optimal magnetic separation.The array’s adaptability allows for seamless integration with separation channels of varying sizes,enabling static separation of whole blood.The platform has a highly flexible processing throughput,spanning from 100μL to 3 mL per separation cycle without sacrificing separation efficiency.A key aspect of this device is its power-free operation throughout the separation process,obviating the complexity of conventional separation devices.Its effectiveness is demonstrated by the extraction of 40μL of plasma from 100μL of rat whole blood within 8 min.The separated plasma proved effective for subsequent analysis of antibody concentration and size in the separated plasma for pharmacokinetic investigations,yielding results on par with those obtained via centrifugation.Furthermore,the device’s high-throughput capability was validated using human whole blood,achieving 3 mL of plasma separation in just 1 min.In a follow-up study on COVID-19 IgG antibody detection,the results matched those from centrifugation.The device demonstrates a separation efficiency of 99.9%for cells larger than 1μm in both rat and human blood samples,with a plasma recovery rate of 72.7%.In summary,our magnetic separation device facilitates rapid plasma extraction from whole blood,with a capacity of up to 3 mL per minute in human blood,without compromising subsequent plasma-based analyses,thereby highlighting its broad applicability across diverse settings.

关 键 词：SEPARATION subsequent SEPARATED 

分 类 号：O65[理学—分析化学]