隔姜灸关元穴治疗寒凝证类原发性痛经大鼠的机制研究  

Study on the mechanism of treating primary dysmenorrhea rats with coagulated cold syndrome by ginger interposed moxibustion at Guan Yuan acupoint

作　　者：邓凯月 邓永志[1] 赵丹[1] 汪音帆 李卓迅 DENG Kaiyue;DENG Yongzhi;ZHAO Dan;WANG Yinfan;LI Zhuoxun(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,长春130117

出　　处：《长春中医药大学学报》2025年第3期278-283,共6页Journal of Changchun University of Chinese Medicine

基　　金：2021年度吉林省科技厅医学(省联合基金白求恩医学项目)(20210101192JC)。

摘　　要：目的 探究隔姜灸关元穴治疗寒凝证类原发性痛经(PD)大鼠的作用及其机制。方法 将40只寒凝证类PD大鼠随机分为空白组(C组)、模型组(M组)、布洛芬阳性药组(Y组)和隔姜灸组(J组),各10只。通过苯甲酸雌二醇和缩宫素以及局部冷冻法建立寒凝证类原发性痛经模型。观测原发性痛经大鼠体质量、肛温检测、扭体次数及评分和子宫指数变化;HE染色检测子宫、卵巢组织病理学变化;检测各组前列腺素E2(PGE2)、前列腺素F2α(PGF2α)、环加氧酶2(COX-2)、β内啡肽(β-EP)、白细胞介素6(IL-6)、白细胞介素1β(IL-1β)各项指标水平。结果 治疗后,各组体质量、肛温、扭体评分、子宫指数、血清中COX-2、PGF2α浓度显著下降(P<0.01),IL-1β浓度显著下降(P<0.05);血清中IL-6、PGE2浓度显著升高(P<0.01),β-EP浓度显著升高(P<0.05)。结论 隔姜灸关元穴可以有效缓解原发性痛经,抑制炎症因子、提高子宫组织PGE2、降低PGF2α可能是其潜在的作用机制。Objective To explore the effect and mechanism of ginger interposed moxibustion at Guanyuan acupoint in the treatment of primary dysmenorrhea(PD)rats with coagulated cold syndrome.Methods 40 PD rats with coagulated cold syndrome were randomly divided into the blank group(group C),the model group(group M),ibuprofen positive drug group(group Y)and ginger interposed moxibustion(group J),with 10 rats in each group.PD model of coagulated cold syndrome using estradiol benzoate,oxytocin,and local freezing method was established.The changes in body mass,anal temperature,twisting frequency and score,and uterine index of rats with PD were observed.HE staining was used to detect pathological changes in uterine and ovarian tissues.The levels of various indicators such as prostaglandin E2(PGE2),prostaglandin F2 alpha(PGF2 alpha),cyclooxygenase-2(COX-2),β-endorphin(β-EP),interleukin-6(IL-6),and interleukin-1β(IL-1β)in each group were detected.Results After treatment,body mass,anal temperature,torsion score,uterine index,concentrations of serum COX-2 and PGF2αin each group significantly decreased(P<0.01),and IL-1βconcentration significantly decreased(P<0.05).Serum IL-6 and PGE2 concentrations significantly increased(P<0.01),andβ-EP concentration significantly increased(P<0.05).Conclusion Ginger interposed moxibustionat at Guanyuan point can effectively relieve PD,inhibit inflammatory factors,increase PGE2 level and decrease PGF2αlevel in uterine tissue,which may be the potential mechanism of action.

关 键 词：隔姜灸 关元穴 寒凝证类 原发性痛经大鼠 

分 类 号：R271.113[医药卫生—中医妇科学]