大孔树脂柱层析法纯化板栗皮结合态多酚的研究  

Purification of Bound Polyphenols from Chestnut Shell by Macroporous Resin Column Chromatography

作　　者：张文 谢辰阳[1,2,3,4] 尚秋 杨芳 ZHANG Wen;XIE Chenyang;SHANG Qiu;YANG Fang(School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China;Key Laboratory for Green Chemical Process of Ministry of Education,Wuhan 430205,China;Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education,Wuhan 430205,China;Hubei Key Laboratory of Novel Reactor and Green Chemical Technology,Wuhan 430205,China)

机构地区：[1]武汉工程大学环境生态与生物工程学院,湖北武汉430205 [2]绿色化工过程教育部重点实验室,湖北武汉430205 [3]磷资源开发利用教育部工程研究中心,湖北武汉430205 [4]新型反应器与绿色化学工艺湖北省重点实验室,湖北武汉430205

出　　处：《化学与生物工程》2025年第3期49-55,共7页Chemistry & Bioengineering

基　　金：湖北省大学生创新创业训练计划项目(S202310490021);武汉工程大学校长基金资助项目(XZJJ2023049);国家自然科学基金青年基金项目(31701604)。

摘　　要：选用5种大孔树脂对板栗外皮和内皮结合态多酚(BP)进行纯化,以吸附率和解吸率为评价指标,对大孔树脂进行了筛选,并对纯化工艺进行了优化。结果表明,采用NKA-9大孔树脂,板栗外皮BP(COSBP)的最佳纯化条件如下:上样浓度为0.22 mg·mL^(-1)、上样pH值为3、上样流速为4.0 BV·h^(-1)、上样体积为26 mL,洗脱液为70%乙醇、洗脱液pH值为7、洗脱流速为3.0 BV·h^(-1)、洗脱体积为28 mL;板栗内皮BP(CISBP)的最佳纯化条件如下:上样浓度为1.33 mg·mL^(-1)、上样pH值为3、上样流速为0.5 BV·h^(-1)、上样体积为8 mL,洗脱液为70%乙醇、洗脱液pH值为7、洗脱流速为0.5 BV·h^(-1)、洗脱体积为33 mL;在上述纯化条件下,COSBP纯度从(1.40±0.04)%升至(24.10±0.35)%,CISBP纯度从(5.00±0.03)%升至(30.50±0.14)%。该工艺绿色环保,为板栗皮BP的进一步利用提供了参考依据。We selected five kinds of macroporous resins to purify the bound polyphenols(BP)from outer shell and inner shell of chestnut.Taking adsorption rate and desorption rate as evaluation indexes,we screened the macroporous resins,and optimized the purification process.The results show that NKA-9 macroporous res-ins are adopted,and the optimal purification conditions of chestnut outer shell BP(COSBP)are as follows:the sample concentration is 0.22 mg·mL^(-1),the sample pH value is 3,the sample flow rate is 4.0 BV·h^(-1),the sample volume is 26 mL;70%ethanol is adopted as the eluent,the eluent pH value is 7,the eluent flow rate is 3.0 BV·h^(-1),and the eluent volume is 28 mL.The optimal purification conditions of chestnut inner shell BP(CISBP)are as follows:the sample concentration is 1.33 mg·mL^(-1),the sample pH value is 3,the sample flow rate is 0.5 BV·h^(-1),the sample volume is 8 mL;70%ethanol is adopted as the eluent,the eluent pH value is 7,the eluent flow rate is 0.5 BV·h^(-1),and the eluent volume is 33 mL.Under above purification conditions,the purity of COSBP increases from(1.40±0.04)%to(24.10±0.35)%,and the purity of CISBP increases from(5.00±0.03)%to(30.50±0.14)%.The process is green and environmentally friendly,which provides a ref-erence for the further utilization of chestnut shell BP.

关 键 词：板栗皮 结合态多酚 大孔树脂 分离纯化 

分 类 号：O658.11[理学—分析化学] R284.2[理学—化学]