龟足碳酸酐酶(CA)基因家族的鉴定及表达分析  

Identification and Expression Analysis of Carbonic Anhydrase(CA)Gene Family in Capitulum mitella

作　　者：宋宇欣 刘凤钰 林岗[1,2] SONG Yu-xin;LIU Feng-yu;LIN Gang(College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China;Fujian Key Laboratory for the Sustainable Utilization of Special Marine Biological Resources,Fuzhou,Fujian 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建省特色海洋生物资源可持续利用重点实验室,福建福州350117

出　　处：《福建农业科技》2025年第1期7-16,共10页Fujian Agricultural Science and Technology

基　　金：中央引导地方科技发展专项(2022L3010)。

摘　　要：碳酸酐酶(carbonic anhydrase,CA)是一类催化二氧化碳和水生成碳酸的酶。为探究CA在龟足壳板钙化过程中的作用,利用生物信息学的方法对龟足CA基因家族成员进行鉴定,分析其编码蛋白的理化性质、亚细胞定位、二级结构、三级结构、保守基序、染色体定位、跨膜结构预测及进化关系,并结合转录组分析龟足CA在不同发育时期的表达模式。结果表明:在龟足基因组中鉴定出10条CA基因家族成员,其分布在6条不同的染色体上。龟足CA基因家族成员编码蛋白的基本理化性质分析表明,蛋白氨基酸数目为108~318个,蛋白相对分子质量为12.10~36.22 kDa,等电点系数范围为5.09~10.68,且均为亲水蛋白;系统发育分析显示在龟足CA蛋白中,CmCA3、CmCA4和CmCA5聚为一支,CmCA6、CmCA7、CmCA8和CmCA9聚为另一支,而CmCA1、CmCA2和CmCA10则分别单独聚为一支;转录组数据分析显示CmCA7、CmCA8、CmCA2、CmCA6和CmCA10在无节幼虫时期表达量相对较低,CmCA5、CmCA3分别在变态中期和稚体时期具有较高的表达量,CmCA4、CmCA1在无节幼虫时期具有较高的表达量,CmCA9在金星幼虫5期表达量增加,随后显著下降。综上所述,研究结果为深入解析CA基因在龟足壳板钙化过程中的调控机制提供理论基础。Carbonic anhydrase(CA)is an enzyme that catalyzes the formation of carbonic acid from carbon dioxide and water.In order to explore the role of CA in the calcification process of Capitulum mitella shell plate,the members of CA gene family of Capitulum mitella were identified by using the bioinformatics method,and their physicochemical properties,subcellular localization,secondary structure,tertiary structure,conserved motifs,chromosomal localization,transmembrane structure prediction and evolutionary relationship of the encoded proteins were analyzed.Then,combined with transcriptome analysis,the expression patterns of CmCA in different developmental stages were also analyzed.The results showed that 10 CA gene family members were identified in the Capitulum mitella genome,which were distributed on six different chromosomes.The analysis on the basic physicochemical properties of the protein encoded by the CA gene family members of Capitulum mitella showed that the number of amino acids of the protein ranged from 108 to 318,with the relative molecular mass of the protein between 12.10 kDa and 36.22 kDa,and the isoelectric point coefficient between 5.09 and 10.68,all of which were hydrophilic proteins.The phylogenetic analysis showed that CmCA3,CmCA4 and CmCA5 were clustered into one branch,while CmCA6,CmCA7,CmCA8 and CmCA9 were clustered into another branch.Meanwhile,CmCA1,CmCA2 and CmCA10 were clustered into independent branch,respectively.The transcriptome data analysis showed that the expression levels of CmCA7,CmCA8,CmCA2,CmCA6,and CmCA10 were relatively low at the stage of nauplius larvae,while CmCA5 and CmCA3 had higher expression levels during the metamorphosis mid-stage and juvenile stage,respectively.CmCA4 and CmCA1 had high expression levels at the stage of nauplius larvae.The expression of CmCA9 increased in the 5th stage of Venus larvae,and then decreased significantly.In summary,the results provided a theoretical basis for the further analysis of the regulatory mechanism of CA gene in the calcification p

关 键 词：龟足 生物信息学 碳酸酐酶(CA)基因家族 表达 

分 类 号：S917.4[农业科学—水产科学]