3种紫淮山茎尖脱毒组培快繁技术研究  

Study on Stem Tip Detoxification and Tissue-cultured Rapid Propagation Technology of Three Kinds of Purple Yam

作　　者：陈丹心 CHEN Dan-xin(Quanzhou Institute of Agricultural Sciences,Quanzhou,Fujian 362212,China)

机构地区：[1]福建省泉州市农业科学研究所,福建泉州362212

出　　处：《福建农业科技》2025年第1期38-43,共6页Fujian Agricultural Science and Technology

基　　金：泉州市科技计划项目(2022N029)。

摘　　要：筛选适合紫淮山茎尖诱导、脱毒苗增殖和生根的培养基以及离体再生苗移栽驯化的基质,为生产上提供健康种苗。以3种紫淮山为供试材料,在MS培养基中添加不同浓度的6-BA、NAA、KT和GA_(3),研究不同植物生长调节剂浓度配比对茎尖诱导、继代增殖及生根的影响,并将离体再生苗移栽到不同配比的进口泥炭土、珍珠岩、椰糠的苗床中,观察不同基质配比对其移栽成活率的影响。结果表明:武夷山紫山药、虎头山紫山药、政和红心薯在添加1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA的MS培养基中茎尖成苗率最高,分别为77.78%、77.78%和71.11%;武夷山紫山药在添加1.0 mg·L^(-1)6-BA+0.5 mg·L^(-1)KT+0.3 mg·L^(-1)NAA+0.1 mg·L^(-1)GA_(3)+0.2 g·L^(-1)AC的MS培养基中增殖系数最高,为4.6,虎头山紫山药及政和红心薯在添加0.5 mg·L^(-1)6-BA+0.2 mg·L^(-1)KT+0.1 mg·L^(-1)NAA+0.1 mg·L^(-1)GA_(3)+0.2 g·L^(-1)AC的MS培养基中增殖系数都为最高,分别为4.83和4.82;武夷山紫山药、虎头山紫山药和政和红心薯在添加0.5 mg·L^(-1)IBA+0.5 mg·L^(-1)NAA+0.5 mg·L^(-1)CCC+0.5 g·L^(-1)AC的MS培养基上根生长情况最好;3种紫淮山在进口泥炭土100%基质中移栽成活率最高,分别为82.7%、80.0%、82.0%。The medium suitable for the stem tip induction,virus-free seedling proliferation and rooting of purple yam and the matrix for the transplantation and domestication of in vitro regenerated seedlings were screened,in order to provide healthy seedlings for the production.Three kinds of purple yam were used as the test materials,and different concentrations of 6-BA,NAA,KT and GA_(3) were added to MS medium,thus to study the effects of different concentration ratios of plant-growth regulator on the stem tip induction,subculture proliferation and rooting.Then,the in vitro regenerated seedlings were transplanted into the seedbeds of imported peat soil,perlite and coconut bran with different ratios,and the effects of different matrix ratios on the survival rate of transplanting were observed.The results showed that the shoot tip seedling rates of Wuyishan purple yam,Hutoushan purple yam and Zhenghe sweet potato were the highest in MS medium supplemented with 1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA,which were 77.78%,77.78%and 71.11%,respectively.The proliferation coefficient of Wuyishan purple yam was the highest in MS medium supplemented with 1.0 mg·L^(-1)6-BA+0.5 mg·L^(-1)KT+0.3 mg·L^(-1)NAA+0.1 mg·L^(-1)GA_(3)+0.2 g·L^(-1)AC,which was 4.6.The proliferation coefficients of Hutoushan purple yam and Zhenghe sweet potato were the highest in MS medium supplemented with 0.5 mg·L^(-1)6-BA+0.2 mg·L^(-1)KT+0.1 mg·L^(-1)NAA+0.1 mg·L^(-1)GA_(3)+0.2 g·L^(-1)AC,which were 4.83 and 4.82,respectively.The root growth of Wuyishan purple yam,Hutoushan purple yam and Zhenghe sweet potato was the best in MS medium supplemented with 0.5 mg·L^(-1)IBA+0.5 mg·L^(-1)NAA+0.5 mg·L^(-1)CCC+0.5 g·L^(-1)AC.The transplanting survival rate of three kinds of purple yam was the highest when transplanted in 100%matrix of imported peat soil,which was 82.7%,80.0%and 82.0%,respectively.

关 键 词：不同品种 紫淮山 茎尖脱毒 组培快繁 

分 类 号：S632.1[农业科学—蔬菜学]