小麦耐盐相关NAC转录因子的生物信息与表达分析  

Bioinformatics and Expression Analysis of Salt Tolerance Related NAC Transcription Factors in Wheat

作　　者：王伟伟[1] 罗政辉 邹景伟 张玉杰 钮力亚[1] 王志 王奉芝[1] 赵振杰 于亮[1] WANG Weiwei;LUO Zhenghui;ZOU Jingwei;ZHANG Yujie;NIU Liya;WANG Zhi;WANG Fengzhi;ZHAO Zhenjie;YU Liang(Cangzhou Academy of Agricultural and Forestry Sciences/Hebei Key Laboratory of Dryland Wheat,Cangzhou,Hebei 061001,China)

机构地区：[1]沧州市农林科学院/河北省旱碱麦重点实验室,河北沧州061001

出　　处：《麦类作物学报》2025年第3期287-299,共13页Journal of Triticeae Crops

基　　金：河北省自然科学基金专项(C2023205050);河北省耐盐碱作物种业科技创新团队项目(23327501D)。

摘　　要：NAC转录因子对植物抗逆境胁迫具有重要作用。为了解小麦耐盐相关NAC转录因子的生物学信息,对耐盐品种沧麦6005和盐敏感品种科农9204进行转录组测序,筛选耐盐相关NAC转录因子,并对其进行生物信息学分析;对供试材料进行盐胁迫处理,提取根部分生组织RNA,反转录为cDNA,对重点候选基因进行qRT-PCR分析。结果表明,共筛选出28个耐盐相关NAC转录因子,其中10个的等电点呈碱性,其余等电点呈酸性,且均为亲水性蛋白;亚细胞定位主要定位在细胞核;蛋白质二级结构以无规则卷曲和α-螺旋为主。经共线性分析,小麦和水稻的NAC转录因子含有多对共线性关系,表明小麦与水稻亲缘关系较近。NAC转录因子启动子区域含有多种与激素响应和逆境胁迫诱导相关顺式作用元件,其中TaNAC24含有顺式调控元件结合位点最多,共有51个。盐胁迫处理后,9个重要基因中,4个基因表达量显著上调,5个基因表达量显著下调,与转录组测序结果一致,这些基因可能介导了小麦的抗盐性。NAC transcription factors play an important role in plant response to stress.In order to further understand the biological functions of salt-tolerant NAC transcription factors in wheat,the transcriptome of salt-tolerant material Cangmai 6005 and salt-sensitive material Kenong 9204 were sequenced.NAC transcription factors related to salt tolerance were screened and bioinformatics analyzed.RT-PCR was used to identify the key candidate genes by reverse transcription of cDNA and RNA extracted from the roots under salt stress.The results showed that among the 28 NAC transcription factors,10 had alkaline isoelectric points and the others had acidic isoelectric points,and all of them were hydrophilic proteins.The protein secondary structure is mainly irregular curl andα-helix.In the collinearity analysis,there were many pairs of collinearity in wheat and rice,indicating that the close relationship between wheat and rice.The promoter region of NAC transcription factor contains many cis-regulatory elements related to hormone response and stress induction,among which TaNAC24 has the most(51)cis-regulatory element binding sites.The results of RT-qPCR showed that the expression levels of four genes were significantly up-regulated and five genes were significantly down-regulated after salt stress treatment,which was consistent with the results of transcriptome sequencing.These genes may mediate the response of wheat to salt stress.

关 键 词：小麦 盐胁迫 NAC 转录因子 生物信息学分析 

分 类 号：S512.1[农业科学—作物学] S330