小麦转录因子TaDL1生物信息学、亚细胞定位与表达分析  

Bioinformatics,Subcellular Localization and Expression Analysis of Wheat Transcription Factor TaDL1

作　　者：沈益庭 刘怡辰 韩俊杰[1] 李卫华[1] SHEN Yiting;LIU Yichen;HAN Junjie;LI Weihua(College of Agriculture,Shihezi University/Key Laboratory of Oasis Ecology Agriculture of Xinjiang Production and Construction Corps,Shihezi,Xinjiang 832000,China)

机构地区：[1]石河子大学农学院/新疆绿洲生态农业兵团重点实验室,新疆石河子832000

出　　处：《麦类作物学报》2025年第3期300-310,共11页Journal of Triticeae Crops

基　　金：新疆生产建设兵团国际科技合作项目(2019BCOO3);石河子大学动植物育种专项(YZZX202003)。

摘　　要：YABBY转录因子家族是一类植物特有的转录因子,在植物叶片生长和花器官建成方面发挥重要作用,其N端具有C2C2型锌指结构域,C端有螺旋-环-螺旋YABBY结构域。本研究以春小麦品种新春9号为材料,克隆到一个YABBY家族的TaDL1基因,其编码区全长603 bp,编码201个氨基酸。经生物信息分析,TaDL1蛋白分子量为22765.21 Da,等电点为8.85,为不稳定亲水性蛋白。通过系统进化树和保守结构域分析,该基因属于YABBY家族的CRC亚家族成员,与水稻OsDL基因亲缘关系较近。经转化烟草进行瞬时表达分析,TaDL1蛋白定位在细胞核上。实时荧光定量分析发现,TaDL1基因在小麦幼穗和叶片中特异性表达,其中在叶片中表达量最高,幼穗中次之。该基因对盐和热胁迫响应,其中对盐胁迫响应最明显且在胁迫后2~24 h内有持续较高的表达量,推测该基因可能参与盐胁迫应答相关的信号途径。The YABBY transcription factor is a type of plant specific transcription factor that plays an important role in leaf growth and floral organogenesis.It has a C2C2 zinc finger domain at the N-terminus and a spiral ring spiral YABBY domain at the C-terminus.This study used a spring wheat variety Xinchun 9 as the material and cloned a TaDL1 gene from the YABBY family,with a total coding region of 603 bp,encoding 201 amino acids.According to bioinformatics analysis,the molecular weight of TaDL1 protein was 22765.21 Da,with an isoelectric point of 8.85,indicating an unstable hydrophilic protein.Through phylogenetic tree and conserved domain analysis,it was found that this gene belonged to the CRC subfamily of the YABBY family and was closely related to the rice OsDL gene.Through transient expression analysis of tobacco,TaDL1 protein was localized in the nucleus.Real time fluorescence quantitative analysis found that TaDL1 was specifically expressed in wheat young ears and leaves,with the highest expression level in leaves and second in young ears.TaDL1 responded to salt and heat stresses,with the most significant response to salt stress and sustained high expression levels within 2-24 h after stress.It is speculated that this gene may be involved in signaling pathways related to salt stress response.

关 键 词：小麦 TaDL1基因 生物信息学 亚细胞定位 表达分析 

分 类 号：S512.1[农业科学—作物学] S330