非人灵长类动物多靶点颅内电极植入的技术方法及效果分析  

Technique and accuracy of multi-target intracranial electrode implantation in non-human primates

作　　者：党圆圆 韩新勇 孙洪吉 何江弘 余山[2] 宋明[2] 张剑宁 DANG Yuanyuan;HAN Xinyong;SUN Hongji;HE Jianghong;YU Shan;SONG Ming;ZHANG Jianning(Department of Neurosurgery,The First Medical Center,Chinese PLA General Hospital,Beijing 100853,China;Institute of Automation,Chinese Academy of Sciences,Beijing 100190,China;College of Future Technology,Peking University,Beijing 100871,China;Department of Neurosurgery,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China)

机构地区：[1]解放军总医院第一医学中心神经外科,北京100853 [2]中国科学院自动化研究所,北京100190 [3]北京大学未来技术学院,北京100871 [4]首都医科大学附属北京天坛医院神经外科,北京100070

出　　处：《空军军医大学学报》2025年第3期299-304,共6页Journal of Air Force Medical University

基　　金：国家自然科学基金面上项目(81771128)。

摘　　要：目的非人灵长类动物可为脑内神经电生理研究提供重要的实验模型。其脑内结构较人类更为精细,颅内电极植入精度要求更高。为确保实验质量,需要优化现有的脑深部电极植入技术。方法同一只猕猴将标准图谱与脑部结构磁共振成像配准,同时结合脑功能影像,确定颅内多靶点感兴趣区,包括中央丘脑、纹状体、额顶皮层、扣带回及海马等。利用手术机器人控制电钻完成颅骨钻孔。通过3次实验,累计植入立体定向脑电图(SEEG)电极29根,脑深部电刺激(DBS)电极4根,并最终植入DBS脉冲发生器。通过术后CT与手术计划融合计算靶点与入点的径向误差确认植入准确性。结果全部电极均按设计路径植入。DBS靶点平均误差为(0.20±0.08)mm,入点平均误差为(0.20±0.06)mm;SEEG靶点平均误差为(0.28±0.13)mm,入点平均误差为(0.22±0.06)mm,其中SEEG靶点误差略高于入点误差。脉冲发生器连接后系统工作正常。手术未出现颅内出血或其他并发症。结论利用动物手术机器人系统及配套的颅骨自动钻孔等功能,可同期安全完成脑内多靶点SEEG及DBS电极植入,并完成脉冲发生器植入,具有较高的精度。Objective Non-human primates serve as crucial experimental models for intracranial neuroelectrophysiological research.Their brain structure is finer than that of human beings,and the accuracy requirement of intracranial electrode implantation is higher.In order to ensure the experimental quality,it is necessary to optimize the existing deep brain electrode implantation technology.Methods In the same macaque,the structural magnetic resonance imaging of the brain was registered with the standard atlas,and combined with functional brain imaging,multiple intracranial target areas of interest were identified,including the central thalamus,striatum,frontal and parietal cortex,cingulate gyrus and hippocampus.The skull drilling was completed by electric drills controlled by a surgical robot.Through three experiments,a total of 29 stereotactic electroencephalography(SEEG)electrodes and 4 deep brain stimulation(DBS)electrodes were implanted in the brain,and finally the DBS pulse generator was implanted.The accuracy of implantation was confirmed by calculating the radial error between the target and the entry point through the fusion of postoperative CT and surgical plan.Results All electrodes were implanted according to the surgical plan,with the mean radial error of the target and entry point was(0.20±0.08)mm and(0.20±0.06)mm for DBS,(0.28±0.13)mm and(0.22±0.06)mm for SEEG.The target point error of SEEG was slightly higher than the entry point error.The pulse generator worked normally after implantation.There was no intracranial hemorrhage or other complications after surgery.Conclusion Using the animal surgical robot system and the automatic drilling technique,the multi-target SEEG and DBS electrodes and pulse generators can be implanted simultaneously,with high accuracy and safety.

关 键 词：脑深部电刺激 立体定向脑电图 猕猴 手术机器人 

分 类 号：R651[医药卫生—外科学] R-332[医药卫生—临床医学]