短肽肠内营养通过保护肠道黏膜屏障功能改善小鼠创伤性脑损伤 神经功能预后  

Short-peptide-based enteral nutrition improves the prognosis of nerve function in mice with traumatic brain injury by protecting intestinal mucosal barrier function

作　　者：王凯 张子文 王亭皓 王嘉辉 葛顺楠 屈延 WANG Kai;ZHANG Ziwen;WANG Tinghao;WANG Jiahui;GE Shunnan;QU Yan(Department of Neurosurgery,Tangdu Hospital,Air Force Medical University,Xi'an 710038,China)

机构地区：[1]空军军医大学唐都医院神经外科,陕西西安710032

出　　处：《空军军医大学学报》2025年第3期318-323,共6页Journal of Air Force Medical University

基　　金：国家自然科学基金(82130038)。

摘　　要：目的探讨早期短肽肠内营养(SPEN)对创伤性脑损伤(TBI)小鼠肠道黏膜屏障功能的保护及对继发性脑损伤的影响。方法选用8~12周龄雄性清洁C57BL/6小鼠,将其分为Sham组、TBI组和TBI+SPEN组。TBI+SPEN组小鼠在造模后立即在饮水中添加短肽粉末(0.4 g/L),其他组在同一过程中接受无菌水。首先分析3组小鼠行为学变化,明确SPEN对TBI小鼠神经功能预后的影响。其次,分析SPEN对TBI小鼠的肠道结构、通透性及血液中脂多糖(LPS)水平的改变,另外检测脑组织含水量、炎症因子水平及小胶质细胞活化情况,明确SPEN对TBI后继发性脑损伤的影响。结果与Sham组相比,TBI组的肠道黏膜屏障结构受到损害,通透性增加,LPS入血、入脑增多(P<0.01),而SPEN的治疗可以有效逆转该损伤,同时减少脑组织内小胶质细胞活化、炎症因子的表达,明显改善神经功能。结论早期SPEN可以通过保护肠道黏膜屏障功能,减少LPS入血、入脑,减轻TBI小鼠继发性脑损伤,改善预后。Objective To investigate the protective effect of short-peptide-based enteral nutrition(SPEN)on intestinal mucosal barrier function and its effect on secondary brain injury in mice with traumatic brain injury(TBI).Methods Male clean-grade C57BL/6 mice aged 8 to 12 weeks were divided into Sham group,TBI group and TBI+SPEN group.Mice in the TBI+SPEN group were given short-peptide powder(0.4 g/L)in drinking water immediately after modeling,while the other groups received sterile water during the same process.Firstly,behavioral changes of the three groups of mice were analyzed to determine the effect of SPEN on the prognosis of nerve function in TBI mice.Secondly,the changes of the structure and permeability in gut and the level of lipopolysaccharide(LPS)in blood were analyzed after TBI mice were given SPEN.In addition,the water content of brain tissues,the level of inflammatory factors and the activation of microglia were detected to determine the effect of SPEN on secondary brain injury after TBI.Results Compared with Sham group,the intestinal mucosal barrier structure was damaged,the permeability was increased,and the entry of LPS into blood and brain was increased in TBI group(P<0.01).However,the damage could be effectively reversed in TBI+SPEN group.At the same time,the activation of microglia and the expression of inflammatory factors in brain tissue were reduced,and the neurological function was significantly improved.Conclusion Early SPEN can protect intestinal mucosal barrier function,reduce the entry of LPS into blood and brain,alleviate secondary brain injury and improve the prognosis of TBI mice.

关 键 词：创伤性脑损伤 肠道屏障 短肽肠内营养 内毒素 

分 类 号：R651.15[医药卫生—外科学]