基于UHPLC-Q-Orbitrap-MS/MS分析2种西洋参炮制品中皂苷类成分  

Analysis of Saponins in Two Processed American Ginseng Products Based on UHPLC-Q-Orbitrap-MS/MS

作　　者：荆野 梁力文 戴雨霖 郑飞 王洋 黄鑫 郭云龙 越皓 JING Ye;LIANG Li-wen;DAI Yu-lin;ZHENG Fei;WANG Yang;HUANG Xin;GUO Yun-long;YUE Hao(Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China;College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,吉林省人参科学研究院,吉林长春130117 [2]长春中医药大学药学院,吉林长春130117

出　　处：《质谱学报》2025年第2期160-174,I0002,共16页Journal of Chinese Mass Spectrometry Society

基　　金：国家自然科学基金(81903778);吉林省科技发展计划(20240305014YY)。

摘　　要：采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-Q-Orbitrap-MS/MS)技术对生晒西洋参和红西洋参中皂苷类成分进行分析,并结合主成分分析、正交偏最小二乘法判别分析和差异成分分析比较2种西洋参炮制品中差异性皂苷。采用Supelco C18色谱柱(3.0 mm×50 mm,2.7μm),以0.1%甲酸水溶液-乙腈作为流动相进行梯度洗脱,在电喷雾离子源正、负离子模式下进行全扫描和二级质谱扫描。本实验鉴定了生晒西洋参和红西洋参中66种皂苷类成分,其中包括31种差异性皂苷。基于实验结果,通过总结4种类型皂苷的质谱碎裂规律,列举了部分皂苷可能的转化过程。该方法准确、高效,可为西洋参及其炮制品的药效物质基础研究和质量评价提供理论依据。Ultra-high performance liquid chromatography-quadrupole-electrostatic field orbital trap high-resolution mass spectrometry(UHPLC-Q-Orbitrap-MS/MS)was used to analyze the saponin components in sun-dried American ginseng and red American ginseng.The principal component analysis,orthogonal partial least squares discriminant analysis,and differential component analysis were employed to compare the differential saponins in these two processed products of ginseng.A Supelco C18 column(3.0 mm×50 mm,2.7μm)was used with 0.1%formic acid in water and acetonitrile as the mobile phase for gradient elution.Full primary MS scan and secondary MS scan were performed by an electrospray ionization source under both positive and negative ion modes.A total of 66 saponin components are identified in sun-dried American ginseng and red American ginseng,among which 31 are differential saponins,including 14 PPD-type,11 PPT-type,and 6 other types.Among the 14 PPD-type differential saponins identified by screening,3 ginsenosides are higher in sun-dried American ginseng,including ginsenoside Rc,malonyl ginsenoside Rb2,and acetyl ginsenoside Rg1,while 11 ginsenosides are higher in red American ginseng,including prototypical saponins,such as ginsenoside Rd,ginsenoside Rb2,and some rare saponins(ginsenoside Rg3,ginsenoside Rs3,ginsenoside Rh2,and others).Among the 11 PPT-type differential saponins,3 ginsenosides are found with high content in sun-dried American ginseng,including malonyl ginsenoside Rg1,malonyl ginsenoside Re and acetyl ginsenoside Re,while 8 ginsenosides are found with high content in red American ginseng,including prototypic saponins,such as ginsenoside Rg2,American ginsenoside R2 and some rare saponins(ginsenoside Rh1,ginsenoside Rg4).Additionally,6 other types of ginsenosides,all of which are rare saponins and more abundant in red ginseng.Acetyl-and malonyl-substituted ginsenosides are more abundant in sun-dried American ginseng,while most of the rare saponins are more abundant in red American ginseng.Based on the results o

关 键 词：高分辨质谱 生晒西洋参 红西洋参 皂苷类成分 

分 类 号：O657.63[理学—分析化学]