固相萃取-超高效液相色谱-串联三重四极杆质谱法测定水体中18种氨基酸  

Determination of 18 Amino Acids in Water by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry

作　　者：梁锦汉 刘则华 刘婉琼[3] 董燕珊 LIANG Jin-han;LIU Ze-hua;LIU Wan-qiong;DONG Yan-shan(School of Environment and Energy,South China University of Technology,Guangzhou 510006,China;Key Laboratory of Pollution Control and Ecological Remediation of Industrial Aggregation Area,Ministry of Education,South China University of Technology,Guangzhou 510006,China;Foshan Sanshui Foshui Water Supply Co.,Foshan 528100,China)

机构地区：[1]华南理工大学环境与能源学院,广东广州510006 [2]华南理工大学,工业聚集区污染控制与生态修复教育部重点实验室,广东广州510006 [3]佛山市三水佛水供水有限公司,广东佛山528100

出　　处：《质谱学报》2025年第2期242-253,I0006,共13页Journal of Chinese Mass Spectrometry Society

摘　　要：在自来水消毒过程中,氨基酸可以与氯、氯胺等消毒剂发生反应,生成具有生物毒性和异味的消毒副产物,需要特别关注。然而,水体中的氨基酸浓度较低,这给检测提出了极高的要求。为此,本研究建立了一种基于固相萃取-超高效液相色谱-串联三重四极杆质谱测定水体中18种氨基酸的方法,并对样品前处理条件和仪器条件进行优化。优化后的最适水样pH 2.8、淋洗液为甲醇-乙酸溶液(1:9,V/V),洗脱液为氨水-甲醇溶液(1:9,V/V),流动相为0.2%甲酸水溶液和含0.2%甲酸的甲醇-乙腈溶液(1:9,V/V)。结果表明,18种氨基酸标准曲线的线性关系良好,相关系数(R^(2))大于0.9913,检出限和定量限分别为0.06~0.23 nmol/L和0.19~0.76 nmol/L,回收率在60.1%~123.2%之间,相对标准偏差为1.7%~18.7%,日间和日内精密度分别为3.7%~7.0%和3.3%~7.1%。采用本方法测定华南地区某自来水厂源水,氨基酸总浓度为300~500 nmol/L,其中,丙氨酸、缬氨酸、天冬氨酸和天冬酰胺的浓度水平占比较高。Amino acids(AAs)are dissolved organic nitrogen,which are widely found in water bodies.During chlorine disinfection,AAs can be converted to potentially biotoxic disinfection byproducts such as haloacetonitrile,halogenated nitroalkanes and haloamides.During the process of chlorine disinfection,chlorine rapidly reacts with the vast majority of free amino acids,particularlyα-amino acids,forming organic monochloramines or dichloramines.These organic monochloramines undergo decarboxylation and dechlorination reactions,resulting in the formation of aldehydes and other odorous disinfection by products.Odor has been an important problem and challenge in the water supply industry,which can cause residents to worry about the safety of tap water,and the rapid warning and the traceability of tap water odor are an effective strategy to deal with the odor of the tap water.Therefore,it is necessary to determine the concentration of AAs in water bodies,especially the tap water.However,the concentration of AAs in water bodies is trace amounts,mostly in the range of nanograms to micrograms per liter,which puts more demand on the development of analytical method.In this work,a method of solid-phase extraction-ultra performance liquid chromatography tandem triple quadrupole mass spectrometry(UPLC-MS/MS)was developed for the determination of 18 amino acids in water bodies,and the sample pretreatment conditions and instrument conditions of UPLC were optimized.The optimal pH value of water sample is 2.8,the eluent of the drip washing is methanol-acetic acid solution(1:9,V/V),the eluent of the purge is ammonia-methanol solution(1:9,V/V),and the mobile phases are 0.2%formic acid in water and 0.2%formic acid in methanol-acetonitrile solution(1:9,V/V).The results showed that the standard curves of this method for 18 AAs have good linearity with the correlation coefficient(R^(2))larger than 0.9913,the limits of detection and the limits of quantification are 0.06-0.23,0.19-0.76 nmol/L,respectively.The recoveries are 60.1%-123.2%,the relative

关 键 词：固相萃取 氨基酸 定量分析 含氮有机物前体 超高效液相色谱-串联质谱(UPLC-MS/MS) 

分 类 号：O657.63[理学—分析化学]