Determination of Perfluorinated Compounds in Milk Tea by High Performance Liquid Chromatography-Tandem Mass Spectrometry  

作　　者：Shu WANG Xianzhi JIANG Juan LUO Qing LI Yaohong LIU Lijun LI Lei YI Dayunzi HUANG 

机构地区：[1]Anhua County Market Supervision Administration,Yiyang 413500,China [2]Hunan GRG Metrology&Test Co.,Ltd.,Changsha 410007,China

出　　处：《Agricultural Biotechnology》2025年第1期64-68,共5页农业生物技术(英文版)

基　　金：Supported by Natural Science Foundation of Hunan Province(2024JJ8266).

摘　　要：[Objectives]This study was conducted to establish a detection method for the simultaneous determination of 18 perfluorinated compounds(PFCs)in milk tea by ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).[Methods]The samples were first subjected to precipitation of proteins by potassium ferrocyanide solution and zinc acetate solution,and then extracted by acetonitrile and detected by LC-MS/MS.Next,sodium chloride was added,and after vortex centrifugation,the acetonitrile layer was dried by blowing with nitrogen.Subsequently,1 ml of methanol was added to prepare a test solution.An ACQUITY UPLC BEH C18(2.6 m,2.1 mm×100 mm)chromatographic column was used for liquid phase separation,and gradient elution was performed using 10 mmol ammonium acetate solution-acetonitrile as the mobile phase.The MS detection adopted the MRM mode for acquisition,positive and negative ion mode switching for simultaneous determination,and external standard method for quantification.[Results]In the linear ranges of the target substances,the correlation coefficients R^(2)were all greater than 0.99.The detection limits of the method was in the range of 0.001-0.05μg/kg,and the quantitation limits were in the range of 0.03-0.20 ng/L.The recovery values ranged from 72.8%to 110.5%.[Conclusions]This method has high sensitivity and good accuracy,and thus strong practical value.

关 键 词：Liquid chromatography-tandem mass spectrometry Milk tea Perfluorinated compound 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]