接种根际促生菌对白花前胡根部品质与根际土壤磷、钾形态的影响  

Effect of different plant growth-promoting Rhizobacteria on transition of phosphorus and potassium element in Rhizosphere soil and roots quality of Peucedanum praeruptorum

作　　者：张萌 王逾涵 刘振宇 钟彦 郭冬琴 周浓 ZHANG Meng;WANG Yuhan;LIU Zhenyu;ZHONG Yan;GUO Dongqin;ZHOU Nong(College of Food and Biological Engineering/Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir Area’s Medicinal Herbs,Chongqing Three Gorges University,Wanzhou 404120,China;College of Pharmacy,Dali University,Dali 671000,China;College of Pharmacy,Chongqing Three Gorges Medical College,Wanzhou 404120,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆万州404120 [2]大理大学药学院,云南大理671000 [3]重庆三峡医药高等专科学校药学院,重庆万州404120

出　　处：《中草药》2025年第3期987-997,共11页Chinese Traditional and Herbal Drugs

基　　金：重庆市教委科学技术研究计划项目(KJQN202001205)。

摘　　要：目的研究不同根际促生菌(plant growth-promoting rhizobacteria,PGPR)对白花前胡Peucedanum praeruptorum根际土壤中磷、钾元素含量、形态转变、元素有效性及其根部药用成分含量的影响,为白花前胡的品质培优提供参考。方法通过盆栽接种实验,将9种不同类型PGPR菌株接种于白花前胡根际土壤,试验设为空白对照(CK)、接种Bacillus thuringiensis(S1)、Paenibacillus amylolyticus(S2)、B.polymyxa(S3)、B.aryabhattai(S4)、B.aryabhattai(S5)、B.cereus(S6)、B.mycoides(S7)、B.proteolyticu(S8)和B.wiedmannii(S9)10个处理组;共生培养9个月后,测定根际土壤中不同形态磷、钾元素及根部中白花前胡甲素、白花前胡乙素含量,分析接种PGPR后白花前胡根部药用成分与根际土壤磷、钾形态含量的变化,并通过相关性与通径分析探讨白花前胡有效成分与根际土壤磷、钾形态的相关性。结果与CK组相比,接种PGPR的9个处理组的白花前胡甲素、白花前胡乙素含量均显著提高,且以S2组效果最为显著,分别较CK组增长了0.513%、0.649%;接种不同PGPR均不同程度增加了白花前胡根际土壤有机磷、有效磷、全磷以及各形态无机磷含量,其中以S5组效果最为显著;接种PGPR可以提高白花前胡根际土壤全钾以及各形态钾素含量,且以S1组效果最为显著;相关性分析表明,各形态无机磷呈极显著正相关,Fe-P、全磷与有效磷呈显著性正相关;全钾、矿物钾、水溶钾均与速效钾呈极显著正相关,缓效钾与速效钾呈显著性正相关,各钾素形态之间呈极显著正相关。通径分析表明,Fe-P、缓效钾对白花前胡甲素呈正效应,有机磷、Ca8-P、有效磷、速效钾、交换钾对白花前胡乙素呈正效应。结论接种不同PGPR能够有效提高白花前胡根际土壤各形态磷、钾素含量,以及白花前胡品质和药用价值,其中以Paenibacillus amylolyticus菌株接种效果最佳。Objectives To study the effect of different plant growth-promoting Rhizobacteria(PGPR)on the content,transformation,and availability of phosphorus and potassium in the rhizosphere soil of Peucedanum praeruptorum,as well as on the quality of its medicinal components,and to provide a reference for optimizing its cultivation.Methods P.praeruptorum was planted in pots and inoculated with nine different PGPR strains.The contents of various forms of phosphorus and potassium in the rhizosphere soil were measured.The experiment was set up as a control(CK),inoculated with Bacillus thuringiensis(S1),Paenibacillus amylolyticus(S2),B.polymyxa(S3),B.aryabhattai(S4),B.aryabhattai(S5),B.cereus(S6),B.mycoides(S7),B.proteolyticu(S8),and B.wiedmannii(S9)ten treatment groups.After a year of inoculation,the transition between different types of phosphorus and potassium element in the rhizosphere soil as well as the contents of praeruptorin A and praeruptorin B in the roots of P.praeruptorum inoculated with PGPR were determined,and the correlation between medicinal ingredients and the forms of phosphorus and potassium element through correlation analysis and path analysis were discussed.Results Compared with CK group,the nine PGPR inoculation groups significantly increased the content of praeruptorin A and praeruptorin B,and the effect of S2 group was the most significant with the increase of praeruptorin A and praeruptorin B by 0.513%and 0.649%respectively.Inoculation with different PGPRs increased the contents of organic phosphorus,available phosphorus,total phosphorus,and various forms of inorganic phosphorus in the rhizosphere soil of P.praeruptorum in varying degrees,and the effect of S5 group was the most significant.Inoculation with PGPRs also increased the contents of total potassium and various types of potassium in the rhizosphere soil of P.praeruptorum,and the effect of S1 group is the most significant.The correlation analysis showed that there was a significant positive correlation between all forms of inorganic phosphoru

关 键 词：根际促生菌 白花前胡 根际土壤 白花前胡甲素 白花前胡乙素 品质 

分 类 号：R286.2[医药卫生—中药学]