红景天内生真菌Penicillium thomii C18中聚酮类次生代谢产物研究  

Secondary metabolites of endophyties Penicillium thomii C18 from Rhodiola rosea

作　　者：董妍 万旭日 刘灵艳 李畅 卢轩[2] 杨异卉[1] 裴月湖 DONG Yan;WAN Xuri;LIU Lingyan;LI Chang;LU Xuan;YANG Yihui;PEI Yuehu(College of Pharmacy,Harbin Medical University,Harbin 150081,China;College of Life Science and Technology,Dalian University,Dalian 116622,China)

机构地区：[1]哈尔滨医科大学药学院,黑龙江哈尔滨150081 [2]大连大学生命健康学院,辽宁大连116622

出　　处：《中草药》2025年第4期1125-1129,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的研究红景天Rhodiola rosea内生真菌Penicillium thomii C18的次生代谢产物。方法采用硅胶和Sephadex LH-20柱色谱及制备型高效液相色谱(HPLC)进行分离纯化,利用核磁共振、质谱、旋光等谱学技术对化合物进行结构鉴定。结果从Penicillium thomii C18培养物的醋酸乙酯萃取物中共分离得到9个聚酮类化合物,包括1个新的化合物与8个已知化合物,分别鉴定为(2'S)-5,7-二羟基-2-(2'-羟基-4'-氧代戊基])-色原酮[(2'S)-5,7-dihydroxy-2-(2'-hydroxy-4'-oxopentyl)-chromone,1]、spirocitromycetin(2)、异决明酮(isotorachrysone,3)、1,6-二羟基-3-甲基-8-甲酯基(口山)酮[1,6-dihydroxy-3-methyl-8-carbomethoxyxanthone,4]、1,5-二羟基-3-甲氧基-7-甲基-蒽-9,10-二酮(1,5-dihydroxy-3-methoxy-7-methyl-anthracene-9,10-dione,5)、9-O-methylneuchromenin(6)、neuchromenin(7)、星形酸(asterric acid,8)、星形酸甲酯(methyl asterrate,9)。结论化合物1为新的聚酮类化合物,命名为拖姆酮A,化合物3和5为首次从青霉属中分离得到。Objective To study the secondary metabolites of the endophyties Penicillium thomii C18 derived from Rhodiola rosea.Methods The secondary metabolites of P.thomii C18 were separated and purified by silica gel,Sephadex LH-20 column chromatography and preparative HPLC.The structures of the isolated compounds were determined by NMR,HR-ESI-MS,polarimetry and etc.Results A total of nine compounds were isolated from the endophytic fungus P.thomii C18.There was one new compound(2'S)-5,7-dihydroxy-2-(2'-hydroxy-4'-oxopentyl)-chromone(1),and eight known compounds spirocitromycetin(2),isotorachrysone(3),1,6-dihydroxy-3-methyl-8-carbomethoxyxanthone(4),1,5-dihydroxy-3-methoxy-7-methyl-anthracene-9,10-dione(5),9-O-methylneuchromenin(6),neuchromenin(7),asterric acid(8),and methyl asterrate(9).Conclusion Compound 1 is a new polyketide named thomiione A.Compounds 3 and 5 are isolated from Penicillium genus for the first time.

关 键 词：红景天 内生真菌 Penicillium thomiiC18 次生代谢产物 聚酮 拖姆酮A 异决明酮 1 5-二羟基-3-甲氧基-7-甲基-蒽-9 10-二酮 

分 类 号：R284.1[医药卫生—中药学]