犬瘟热病毒核酸检测技术在临床诊断中的应用研究  

Application of Canine Distemper Virus Nucleic Acid Detection Technology in Clinical Diagnosis

作　　者：阿曼吐尔·阿黑哈提 Amantul Ahehati(Ili Vocational and Technical College,Ili Xinjiang 835100,China)

机构地区：[1]伊犁职业技术学院,新疆伊犁835100

出　　处：《现代畜牧科技》2025年第2期104-106,共3页Modern Animal Husbandry Science & Technology

基　　金：2022年自治区牧区地方肉羊品种选育推广技术体系任务书;牧区地方肉羊品种选育推广技术体系(XJMQ-22)。

摘　　要：犬瘟热是由犬瘟热病毒引起的一种高度接触性传染病,临床诊断准确性直接关系到患犬预后。该文从定义和原理等方面概述了犬瘟热病毒核酸检测技术,分析该技术在临床诊断中存在的样本采集处理不当导致漏检、引物探针设计不合理影响特异性、检测方法选择不当降低灵敏度、联合检测和综合诊断意识不足等缺点,并提出了规范样本采集处理流程、优化引物探针设计、合理选择检测方法、加强联合检测和综合诊断等优化策略建议,以期为犬瘟热诊断和防控提供参考。Canine distemper is a highly contagious disease caused by canine distemper virus,and the accuracy of clinical diagnosis is directly related to the prognosis of infected dogs.This paper summarizes canine distemper virus nucleic acid detection technology from the aspects of definition,principle and applicable diseases,and analyzes the shortcomings of this technology in clinical diagnosis,such as improper sample collection and processing leading to missed detection,unreasonable primers and probes design affecting specificity,improper selection of detection methods reducing sensitivity,lack of awareness of joint detection and comprehensive diagnosis.In order to provide reference for diagnosis and control of canine distemper,some optimization strategies were proposed,such as standardizing sample collection and processing process,optimizing primers and probes design,rationally selecting detection methods,strengthening joint detection and comprehensive diagnosis.

关 键 词：犬瘟热病毒 核酸检测 临床诊断 优化策略 

分 类 号：S858.292[农业科学—临床兽医学]