miRNA表达水平检测技术研究进展  

Advances in the Detection Technique of miRNA Expression Level

作　　者：周兆瑞 徐梦思 甘尚权 ZHAO Zhaorui;XU Mengsi;GAN Shangquan(College of Animal Science,Xinjiang Agricultural University,Urumqi Xinjiang 830000,China;Institute of Animal husbandry and Veterinary Sciences,Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi Xinjiang 832000,China;Shihezi University College of Animal Science and Technology Shihezi Xinjiang 832000,China;Binhai Agricultural College,Guangdong Ocean University,Zhanjiang 524008,China)

机构地区：[1]新疆农业大学动物科学学院,新疆乌鲁木齐830000 [2]新疆农垦科学院畜牧兽医研究所,新疆石河子832000 [3]石河子大学动物科技学院,新疆石河子832000 [4]广东海洋大学滨海农业学院,广东湛江524008

出　　处：《现代畜牧科技》2025年第2期127-131,共5页Modern Animal Husbandry Science & Technology

基　　金：国家自然科学基金面上项目“miR-SELENBP1介导KRTAP6家族基因表达影响羊毛周期性生长与细度性状的机制研究”(32472889);兵团英才青年项目(2023-2025)。

摘　　要：miRNA(microRNA)是一类由为21~23个核苷酸组成的小型非编码RNA分子,它们主要通过靶mRNA序列中3′非编码区(3′UTR)相互结合,进而调控靶基因的表达,一般通过抑制的方式影响靶基因的翻译。目前,miRNA已被证实在机体的各种生命活动过程中发挥了关键的调控作用。因此,研发具有高特异性和高灵敏度的miRNA检测技术尤为重要。该文将围绕Northern blot(Northern印记)、实时荧光定量PCR(qRT-PCR)、荧光原位杂交(FISH)、miRNA测序法进行综述。miRNAs(microRNAs)are small non-coding RNA molecules approximately 21 to 23 nucleotides in length.They primarily regulate target gene expression by binding to the 3′untranslated region(3′UTR)of target mRNA sequences,often suppressing the translation process.Extensive research has demonstrated that miRNAs play crucial regulatory roles in various biological activities.Consequently,developing miRNA detection technologies with high specificity and sensitivity has become a critical focus.This review provides a comprehensive overview of current miRNA detection methods,including Northern blot,quantitative real-time PCR(qRT-PCR),fluorescence in situ hybridization(FISH),and miRNA sequencing.

关 键 词：miRNA检测 Northern blot QRT-PCR FISH miRNA测序法 

分 类 号：Q522[生物学—生物化学]