超高效液相色谱-三重四极杆-线性离子阱质谱快速检测动物源性食品中3种胃动力药物残留  

Determination of Three Supplementary Chemical Drugs in Food Products of Animal Origin by UPLC-MS/MS-QTRAP

作　　者：张宪臣 胡仪光 齐鹤鸣 李勇 梁娜娜 ZHANG Xian-Chen;HU Yi-Guang;QI He-Ming;LI Yong;LIANG Na-Na(Technology Center of Zhongshan Customs,Zhongshan 528403;Science and Technology Research Center of China Customs,Beijing 100026)

机构地区：[1]中山海关技术中心,中山528403 [2]中国海关科学技术研究中心,北京100026

出　　处：《中国口岸科学技术》2025年第2期71-79,共9页China Port Science and Technology

基　　金：拱北海关科技项目(2023GK016)。

摘　　要：本研究建立了超高效液相色谱-三重四极杆-线性离子阱质谱法快速检测畜肉、鱼肉和动物内脏等动物源性食品中3种胃动力药物(多潘立酮、莫沙必利和西沙必利)的方法。样品经乙酸乙酯提取、浓缩、复溶,采用20 mmol/L乙酸铵水溶液和甲醇溶液梯度洗脱,Agilent poroshell C_(18)色谱柱(100 mm×3.0 mm,2.7μm)进行分离;质谱采用电喷雾离子源(ESI)正离子检测,多重反应检测(MRM)模式下进行内标法定量。结果显示,目标化合物多潘立酮、莫沙必利和西沙必利在线性范围内线性关系良好,相关系数(r)≥0.9991,检出限为0.08μg/kg,定量限为0.20μg/kg,实际空白样品加标回收率在80.5%~110.5%之间,变异系数为0.83%~7.55%。结果表明,该方法检测效率高、检测结果准确,适用于动物源性食品中3种胃动力药物的快速检测。This study established an ultra-high performance liquid chromatography tandem-linear ion trap mass spectrometry(UPLC-MS/MS-QTRAP)method for the determination of three supplementary chemical drugs(domperidone,mosapride and cisapride)in food products of animal origin,including meat from livestock,fish,and animal organs.The samples were extracted with ethyl acetate,concentrated and re-dissolved.The separation of the three target compounds was performed on an Agilent poroshell C_(18) column(100 mm×3.0 mm,2.7μm),using a gradient elution of methanol and 20 mmol/L amine acetate solution as the mobile phase.The target compounds were detected in positive electrospray ionization(ESI)using multiple reaction monitoring(MRM)mode,and quantified using the internal standard method.The result showed that the calibration curves of the three supplementary chemical drugs showed good linearity(r≥0.9991).The detection limits were 0.08μg/kg,and the quantitation limits were 0.20μg/kg.The recoveries of actual blank spiked sample ranged from 80.5%to 110.5%,with a coeficient of variation(CV)between 0.83%and 7.55%.This method has higher analysis efficiency,higher sensitivity and accuracy,making it suitable for the rapid detection of these three supplementary chemical drugs in food products of animal origin.

关 键 词：超高效液相色谱-三重四极杆-在线离子阱质谱 动物源性食品 胃动力药物 检测 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]