融合蛋白AL-3AA对耐碳青霉烯肺炎克雷伯菌的体外抗菌活性研究  

Study on the in vitro antibacterial activity of fusion protein AL-3AA against carbapenem-resistant Klebsiella pneumoniae

作　　者：朱丽娜[1] 周俊秀 郑永祥 余蓉[2] 邓劲[1] 何超[1] ZHU Lina;ZHOU Junxiu;ZHENG Yongxiang;YU Rong;DENG Jin;HE Chao(West China Hospital of Sichuan University,Chengdu,Sichuan,610041,P.R.China;West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西医院,四川成都610041 [2]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2025年第1期14-16,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川省科学技术厅重点研发项目(2021YFS0183)。

摘　　要：目的评价融合蛋白AL-3AA对耐碳青霉烯肺炎克雷伯菌(CRKP)的体外抗菌活性。方法通过最低抑菌浓度(MIC)、抗菌活性测定、时间杀菌曲线和扫描电镜分析AL-3AA的体外抗菌活性。结果AL-3AA对CRKP的MIC为6.5μmol·L^(-1),60 min内可使菌量降低3.74个对数单位,杀菌能力呈时间依赖性,可破坏细菌细胞壁。结论AL-3AA对CRKP具明显的体外抗菌活性。OBJECTIVE To evaluate the in vitro antibacterial activity of fusion protein AL-3AA against carbapenem-resistant Klebsiella pneumoniae(CRKP).METHODS The in vitro antibacterial activity of AL-3AA was analyzed by minimum inhibitory concentrations(MIC),antibacterial activity assay,time-kill curve and scanning electron microscope.RESULTS The MIC of AL-3AA against CRKP was 6.5μmol·L^(-1).AL-3AA reduced the bacterial load by 3.74 logarithmic units in 60 minutes,showed time-dependent antibacterial ability,and could destroy the cell wall of the bacteria.CONCLUSION AL-3AA showed significant in vitro antibacterial activity against CRKP.

关 键 词：裂解酶 抗菌肽 融合蛋白 AL-3AA 耐碳青霉烯肺炎克雷伯菌 抗菌活性 最低抑菌浓度 时间杀菌曲线 

分 类 号：R96[医药卫生—药理学] R915[医药卫生—药学]