重组贻贝粘蛋白凝胶治疗小鼠特应性皮炎的机制研究  

Study on the mechanism of recombinant mussel mucin gel on atopic dermatitis in mice

作　　者：韩明雨 李雪[1] 杨简宁 张恒 彭芙 HAN Mingyu;LI Xue;YANG Jianning;ZHANG Heng;PENG Fu(Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry,Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology,West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China)

机构地区：[1]四川大学华西药学院四川省植物来源药物工程实验室、四川省小分子药物精准化工程技术研究中心靶向药物及释药系统教育部重点实验室,四川成都610041

出　　处：《华西药学杂志》2025年第1期41-44,共4页West China Journal of Pharmaceutical Sciences

基　　金：四川省杰出青年科技人才项目(2023NSFSC1928)。

摘　　要：目的研究重组贻贝粘蛋白凝胶(RMMG)的止痒效果及机制。方法研究长期使用RMMG对4-氨基吡啶致小鼠特应性皮炎的止痒作用,以苏木精-伊红和免疫组化染色法观察小鼠皮损组织的炎症变化,酶联免疫吸附测定法检测小鼠体内组织的炎症变化;以Western blotting和实时荧光定量PCR实验检测RMMG的作用机制。结果与模型组比较,RMMG组小鼠的搔抓次数明显减少,小鼠皮肤炎性浸润明显改善,皮肤组织中5-羟色胺受体2A的含量显著减少,血清中白介素-6(IL-6)、肿瘤坏死因子α(TNF-α)和组胺的水平明显降低。RMMG可能通过抑制核因子κB(NF-κB)通路发挥抗瘙痒的作用。结论RMMG可通过组胺/IL-6/TNF-α和NF-κB通路来抑制4-氨基吡啶致小鼠皮肤急性瘙痒。OBJECTIVE To study the antipruritic effect and mechanism of recombinant mussel mucin gel(RMMG).METHODS The antipruritic effect of RMMG on mice with atopic dermatitis induced by 4-aminopyridine was studied.The changes of skin inflammation in mice were observed using Hematoxylin-eosin and 5-hydroxytryptamine receptor 2A immunohistochemical staining.Tissue inflammation changes in mice were detected by enzyme-linked immunosorbent assay.Western blotting and Real-time quantitative polymerase chain reaction were used to determine the mechanism.RESULTS Compared with the model group,the scratching frequency of mice in RMMG groups was significantly reduced,the skin inflammatory infiltration showed a significant improvement,and the serum levels of IL-6,TNF-αand histamine were significantly reduced.RMMG inhibited pruritus in mice by inhibiting NF-κB pathway.CONCLUSION RMMG can inhibit acute pruritus induced by 4-aminopyridine through histamine/IL-6/TNF-αand NF-κB pathways.

关 键 词：重组贻贝粘蛋白凝胶 特应性皮炎 瘙痒 组胺 4-氨基吡啶 核因子κB通路 

分 类 号：R96[医药卫生—药理学]