Effects of direct moxibustion on antigen-presenting cells in gastric tissue of rat models with gastric cancer:Understanding the immunological mechanisms  

记笔记直接灸对胃癌大鼠胃组织抗原提呈细胞的影响及其免疫机制探讨

作　　者：Tian-yu GAO Fei-cheng ZHANG Ru-fang PAN Shu-ya MA Xiao-qi ZHANG Li-jia PAN Chun-sheng JIA 高田宇;张飞程;潘茹芳;马舒雅;张晓琪;潘丽佳;贾春生(河北中医学院针灸推拿学院,石家庄050200;河北省刺灸法效应特异性重点研究室,石家庄050091)

机构地区：[1]School of Acupuncture,Moxibustion and Tuina,Hebei University of Chinese Medicine,Shijiazhuang 050200,China [2]Hebei Key Laboratory of Acupuncture and Moxibustion Specificity Effects,Shijiazhuang 050091,China

出　　处：《World Journal of Acupuncture-Moxibustion》2025年第1期66-74,共9页世界针灸杂志(英文版)

基　　金：Supported by the National Natural Science Foundation of China:81173342;Scientific Research Ability Improvement Projects of Hebei College of Traditional Chinese Medicine(now known as Hebei University of Chinese Medicine):KTZ2019012;Scientific Research Project of Hebei Province Administration of Traditional Chinese Medicine:2022361.2021095。

摘　　要：Objective:The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N-methyl-N’-nitro-N-nitrosoguanidine(MNNG)were evaluated.Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated.The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions,thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development.Methods:Sixty healthy male Wistar rats were randomly divided into four groups:Control,control+moxibustion,model,and moxibustion groups.A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG,with a dose of 1 mL/100 g body weight,once daily for 16 weeks.The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling,continuing for 16 weeks.After the experiment,gastric tissue was collected,and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining.Immunohistochemistry(IHC)and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues.Enzyme-linked immunosorbent assays(ELISA)were used to measure the levels of interleukin-12(IL-12),interferon-gamma(IFN-γ),and tumor necrosis factor-beta(TNF-β)in rat serum.Results:Upon macroscopic observation,the gastric mucosa of rats in the control and control+moxibustion groups appeared uniformly red,with a glossy mucosal surface,normal gastric wall elasticity,and clear,regular mucosal folds,without hyperplasia or bleeding points.In the model group,the gastric mucosa was reduced in volume,the gastric wall thinned,elasticity decreased,mucosal folds were disordered,and yellow-white cauliflower-like lesions and yellow-brown hyperkeratosis were observed.In the moxibustion group,the gastric mucosa showed decreased elasticity,with disordere

关 键 词：Gastric cancer Direct moxibustion ZUSANLI St 36 Antigen-presenting cells Immunological mechanism 

分 类 号：R24[医药卫生—中医临床基础]