非洲马瘟病毒和西尼罗病毒多酶恒温核酸快速扩增检测方法的建立  

Establishment of MIRA Detection Method for African Horse Sickness Virusand West Nile Virus

作　　者：钱佳豪 刘丹[2] 周师众 张博源 高建帅 许霄峰 李慧彤 丁家波[2] 王春凤[1] 沈青春 QIAN Jia-hao;LIU Dan;ZHOU Shi-zhong;ZHANG Bo-yuan;GAO Jian-shuai;XU Xiao-feng;LI Hui-tong;DING Jia-bo;WANG Chun-feng;SHEN Qing-chun(College of Veterinary Medicine,Jilin Agricultural University,Changchun,Jilin,130118,China;Key Laboratory of Animal Biosafety Risk Prevention and Control(North),Beijing Institute of Animal Husbandry and Veterinary Medicine of CAAS,Beijing,100193,China)

机构地区：[1]吉林农业大学动物医学院,吉林长春130118 [2]中国农业科学院北京畜牧兽医研究所,农业农村部动物生物安全风险预警及防控重点实验室(北方),北京100193

出　　处：《动物医学进展》2025年第3期12-16,共5页Progress In Veterinary Medicine

基　　金：国家重大外来动物疫病阻断与防控技术研发项目(2022YFD1800500)。

摘　　要：为了建立一种方便快捷的非洲马瘟病毒(AHSV)和西尼罗病毒(WNV)的检测方法,将GenBank中非洲马瘟病毒VP7序列以及近3年世界范围内流行的西尼罗病毒序列进行比对,选择保守区域,并按照需求设计引物与探针。通过反应条件的优化,以及特异性与敏感性试验,建立了一种能够同时检测非洲马瘟病毒和西尼罗病毒的多酶恒温核酸快速扩增(MIRA)方法。结果表明该方法操作简便、灵敏度高、特异性强,反应时间仅需20 min。该检测方法可应用于非洲马瘟和西尼罗热的快速诊断和现场监测,为疫情控制和流行病学研究提供技术支持。In order to establish a convenient and fast detection method for African horse sickness virus and West Nile virus.Compared the African horse sickness virus VP7 sequence and the West Nile virus sequence that has been circulating worldwide in the past three years in GenBank,the conservative regions were selected to design primers and probes according to requirements.A multienzyme isothermal rapid amplification(MIRA)method was established to simultaneously detect African horse sickness virus and West Nile virus through optimization of reaction conditions and specificity and sensitivity tests.The experimental results show that this method is easy to operate,highly sensitive,and has strong specificity,with a reaction time of only 20 minutes.This detection method can be applied to the rapid diagnosis and on-site monitoring of African horse sickness and West Nile fever,providing technical support for epidemic control and research.

关 键 词：非洲马瘟病毒 西尼罗热病毒 多酶恒温快速扩增技术 

分 类 号：S852.65[农业科学—基础兽医学]