黄芩泻白散中8种活性成分的高效液相色谱法测定  

Determination of Eight Active Components in Huangqin Xiebai San by High Performance Liquid Chromatography

作　　者：张文飞 纪鹏[1] 董嘉琪 买占海 韩罗霞 何磊 华永丽[1] 袁子文[1] 王秀琴 魏彦明[1] ZHANG Wen-fei;JI Peng;DONG Jia-qi;MAI Zhan-hai;HAN Luo-xia;HE Lei;HUA Yong-li;YUAN Zi-wen;WANG Xiu-qin;WEI Yan-ming(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu,730070,China;Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia,750002,China)

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]宁夏农林科学院动物科学研究所,宁夏银川750002

出　　处：《动物医学进展》2025年第3期93-97,共5页Progress In Veterinary Medicine

基　　金：宁夏重点研发项目(2023BCF01039);国家自然科学基金(32260899,32060812);甘肃农业大学青年导师基金资助项目(GAU-QDFC-2021-04);财政部和农业农村部:国家现代农业产业技术体系(CARS-37)。

摘　　要：建立一种同时测定黄芩泻白散中8种活性成分含量的方法。采用高效液相色谱法(HPLC)同时测定黄芩泻白散中桑皮苷A(Mulberroside A)、绿原酸(Chlorogenic acid)、黄芩苷(Baicalin)、槲皮素(Quercetin)、汉黄芩苷(Wogonoside)、黄芩素(Baicalein)、甘草酸(Glycyrrhizic acid)和汉黄芩素(Wogonin)的含量。用Agilent Zorbax SB C18色谱柱(250 mm×4.6 mm,5μm);0.1%磷酸(A)-乙腈(B),梯度洗脱,柱温为29℃,检测波长为250 nm,体积流量为0.8 mL/min,进样量20μL。结果表明,8种活性成分在各自相应浓度范围内r值均大于0.9996,线性关系良好。测得桑皮苷A、绿原酸、黄芩苷、槲皮素、汉黄芩苷、黄芩素、甘草酸和汉黄芩素的加样回收率分别为98.38%、97.90%、98.56%、91.38%、99.20%、99.71%、100.28%和106.18%,RSD值均小于5%,平均含量分别为38.56、218.53、2493.01、16.54、242.72、1839.74、90.54、343.95μg/g。说明该方法重复性、稳定性良好,准确灵敏,可用于黄芩泻白散活性成分的测定和质量控制,为研发新兽药奠定基础。A method was established for the simultaneous determination of the eight active components in Huangqin Xiebai San.High-performance liquid chromatography(HPLC)was used for simultaneous determination of mulberroside A,chlorogenic acid,baicalin,quercetin,wogonoside,baicalein,glycyrrhizic acid and wogonin in Huangqin Xiebai San.Agilent Zorbax SB C18 column(250 mm×4.6 mm,5μm)was used,with 0.1%phosphoric acid(A)-acetonitrile(B),gradient elution,column temperature at 29℃,detection wavelength at 250 nm,volumetric flow rate of 0.8 mL/min and injection volume of 20μL.The r-values of the eight active components in their respective corresponding concentration ranges were all greater than 0.9996,with good linear relationships.The average recovery rates of mulberroside A,chlorogenic acid,baicalin,quercetin,wogonoside,baicalein,glycyrrhizic acid and wogonin were measured to be 98.38%,97.90%,98.56%,91.38%,99.20%,99.71%,100.28%and 106.18%,respectively,with RSD values less than 5%,and the average contents were 38.56,218.53,2493.01,16.54,242.72,1839.74,90.54,343.95μg/g.The results showed that the method is reliable,reproducible and accurate.It can be used for the determination and quality control of the active components of Huangqin Xiebai San,laying the foundation for the research and development of new veterinary drugs.

关 键 词：黄芩泻白散 活性成分 测定 高效液相色谱法 

分 类 号：S853.7[农业科学—临床兽医学]