基于HPLC图谱和一测多评法的沙棘颗粒质量评价研究  

Study on quality evaluation of Seabuckthorn granules based on HPLC chromatogram and one test and multiple evaluation method

作　　者：左玲燕 张少杰 ZUO Lingyan;ZHANG Shaojie(Guangzhou Institute for Drug Control,Guangzhou Guangdong 510000,China;Guangzhou University of Traditional Chinese Medicine,Guangzhou Guangdong 510000,China)

机构地区：[1]广州市药品检验所一分所,广东广州510000 [2]广州中医药大学,广东广州510000

出　　处：《天津药学》2025年第1期7-11,共5页Tianjin Pharmacy

摘　　要：目的旨在构建沙棘颗粒的高效液相色谱(HPLC)指纹图谱,并通过一测多评(QAMS)技术对其中的关键活性成分进行定量分析,实现对沙棘颗粒质量的系统评价。方法采用HPLC法建立沙棘颗粒指纹图谱,并通过相似度评价、主成分分析(PCA)对指纹图谱进行分析;选取槲皮素为内参物,构建山柰素、异鼠李素含量的QAMS模型,并与外标法(ESM)进行结果对比。结果9批沙棘颗粒的HPLC指纹图谱中有13个共有峰,识别出槲皮素、山柰素、异鼠李素这三种成分,相似度为0.92~1.00。PCA分析显示,沙棘颗粒供试品主要差异体现在峰1(槲皮素)、峰2(山柰素)、峰3(异鼠李素)。此外,对于3种成分的分析结果,QAMS法与ESM法测定值无显著差异。结论研究建立的HPLC指纹图谱及QAMS法操作简便、效率高、准确性好、耐用性强,为沙棘颗粒的质量评价提供了可靠的参考依据。Objective The aim of this study was to construct high performance liquid chromatography(HPLC)fingerprint of Seabuckthorn granules,and quantitatively analyze the key active components by one test and multiple evaluation(QAMS)technique,so as to achieve systematic evaluation of seabuckthorn granules quality.Methods The fingerprints of Seabuckthorn granules were established by HPLC,and analyzed by similarity evaluation and principal component analysis(PCA).Using quercetin as internal reference,the QAMS model of kaempferol and isorhamnetin content was constructed,and the results were compared with those of external standard method(ESM).Results There were 13 common peaks in the HPLC fingerprints of 9 batches of Seabuckthorn granules,and quercetin,kaempferol and isorhamnetin were identified with similarity ranging from 0.92 to 1.0.PCA analysis showed that the main differences of sea buckthorn granules were peak 1(quercetin),peak 2(kaempferol)and peak 3(isorhamnetin).In addition,there was no significant difference between QAMS method and ESM method in the analysis results of the three components.Conclusion The HPLC fingerprint and QAMS method established in this study are simple,efficient,accurate and durable,and provide a reliable reference for the quality evaluation of Seabuckthorn granules.

关 键 词：高效液相色谱 指纹图谱 沙棘颗粒 一测多评法 质量评价 

分 类 号：O657.7[理学—分析化学] R286[理学—化学]