应用转录组测序技术分析干旱胁迫下大豆落花落荚的调控基因  

Application of transcriptome sequencing technology to analyze the regulatory genes of soybean flower and pod under drought stress

作　　者：卢文田 曹凤鸣 周静文[1] 全乐 冯瑞 武小霞 柏锡[1] LU Wentian;CAO Fengming;ZHOU Jingwen;QUAN Le;FENG Rui;WU Xiaoxia;BAI Xi(School of Life Sciences,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2024年第8期14-27,共14页Journal of Northeast Agricultural University

基　　金：国家自然科学基金区域创新发展联合基金项目(U23A20192)。

摘　　要：光合作用和ABA在大豆生长发育中发挥重要作用,光合作用为大豆提供必需的有机物质,ABA则参与调节植物对逆境的影响,两者相互作用,共同影响大豆落花落荚。利用转录组测序技术,分析大豆一节期(V1)、二节期(V2)、三节期(V3)、始花期(R1)受干旱胁迫时叶片中转录组变化,从光合作用和ABA相关角度寻找潜在的调控大豆落花落荚的关键基因。转录组数据揭示淀粉和蔗糖合成相关基因、ABA合成、代谢和信号转导相关基因,在干旱胁迫下表达水平变化,筛选到在4个时期均影响干旱的光合作用途径相关基因Glyma.08G082900m荚的核心转录因子,如MYB相关转录因子(Glyma.20G186500、Glyma.10G006600)、MYB转录因子(Glyma.09G038900、Glyma.03G125000)、bZIP转录因子(Glyma.14G217200、Glyma.04G039300)、Dof转录因子(Glyma.05G025900)。Photosynthesis and ABA play important roles in the growth and development of soybeans.Photosynthesis provides the necessary organic matter for soybeans,while ABA is involved in regulating the plant's response to stress.The interaction between the two jointly affects the phenomenon of flower and pod abscission in soybeans.This study used transcriptome sequencing technology to analyze the transcriptome changes in the leaves of soybeans during the first node stage(V1),the second node stage(V2),the third node stage(V3),and the initial flowering stage(R1)under drought stress,looking for key genes that regulated flower and pod abscission in soybeans under drought stress from the perspectives of photosynthesis and ABA pathways.The transcriptome data revealed changes in the expression levels of genes related to starch and sucrose synthesis,as well as genes related to ABA synthesis,metabolism and signal transduction under drought stress.Genes related to the photosynthesis pathway that responded to drought in all four stages were identified,such as Glyma.08G082900 and Glyma.16G162600,as well as genes significantly related to the ABA metabolic pathway,such as Glyma.14G056300.Potential core transcription factors that regulated flower and pod abscission under drought stress were also identified,including MYB-related transcription factors(Glyma.20G186500 and Glyma.10G006600),MYB transcription factors(Glyma.09G038900 and Glyma.03G125000),bZIP transcription factors(Glyma.14G217200 and Glyma.04G039300),and Dof transcription factors(Glyma.05G025900).

关 键 词：大豆 干旱 落花落荚 转录组测序 

分 类 号：S565.1[农业科学—作物学]