万氏愈瘫汤对缺血性脑卒中大鼠的治疗作用及机制探讨  

Therapeutic Effect and Mechanism of Wanshi Yutan Decoction on Ischemic Stroke in Rats

作　　者：潘微[1] 王非[1] 张京兰[1] 刘江华[1] 卢菁 杨晶[1] 王翠[1] 鲁铭[1] PAN Wei;WANG Fei;ZHANG Jinglan;LIU Jianghua;LU Jing;YANG Jing;WANG Cui;LU Ming(Wuhan Traditional Chinese Medicine Hospital,Wuhan 430014,China)

机构地区：[1]武汉市中医医院,湖北武汉430014

出　　处：《中医药学报》2025年第3期32-36,共5页Acta Chinese Medicine and Pharmacology

基　　金：湖北省卫生健康委员会中医药科研面上项目(ZY2019M035);湖北省卫生和计划生育委员会科研项目(WZ21C31)。

摘　　要：目的:研究万氏愈瘫汤对缺血性脑卒中大鼠的作用,并探讨其可能机制。方法:将42只SD大鼠随机分为7组,正常组、假手术组、模型组、万氏愈瘫汤低(12.60 g·kg^(-1)·d^(-1))、中(25.20 g·kg^(-1)·d^(-1))、高(50.40 g·kg^(-1)·d^(-1))剂量组和阳性药物(9.45 mg·kg^(-1)·d^(-1)尼莫地平)组,每组6只。构建缺血性脑卒中大鼠模型并分组给药,观察大鼠一般行为,并进行神经功能损伤评分(MNSS)。收集大鼠脑组织,TTC染色观察脑组织梗死区域,计算梗死率。TUNEL染色观察大鼠海马组织细胞凋亡水平。Western blot法检测大鼠脑梗死组织中活化型半胱氨酸天冬氨酸蛋白酶-3(Cleaved-caspase-3)、Bcl-2和Bax表达水平。结果:模型组大鼠出现毛发粗糙、口角流涎、精神状态差等症状,MNSS评分显著升高(P<0.05),海马组织中细胞凋亡增加(P<0.05),Cleaved-caspase-3和Bax表达水平显著升高(P<0.05),Bcl-2表达水平显著降低(P<0.05)。与模型组比较,万氏愈瘫汤组大鼠症状减轻(P<0.05),MNSS评分降低(P<0.05),海马组织中细胞凋亡减少(P<0.05),Cleaved-caspase-3和Bax表达水平显著降低(P<0.05),Bcl-2表达水平显著升高(P<0.05)。结论:万氏愈瘫汤可改善缺血性脑卒中大鼠症状,其作用机理可能与减少脑组织细胞凋亡相关。Objective:To investigate the effects of Wanshi Yutan Decoction on ischemic stroke in rats and explore its potential mechanisms.Methods:Forty-two Sprague-Dawley(SD)rats were randomly divided into seven groups:normal,sham-operated,model,low-dose,medium-dose(25.20 g·kg^(-1)·d^(-1))and high-dose(50.40 g·kg^(-1)·d^(-1))Wanshi Yutan Decoction(12.60 g·kg^(-1)·d^(-1))groups,and positive drug(nimodipine 9.45 mg·kg^(-1)·d^(-1))group,with six rats in each group.An ischemic stroke rat model was established,and rats were administered treatment accordingly.General behaviors were observed,and neurological function was assessed using the Modified Neurological Severity Score(mNSS).Brain tissue samples were collected,and TTC staining was used to observe infarct regions and calculate the infarction rate.Apoptosis in hippocampal tissue was evaluated using TUNEL staining.Western blot analysis was used to measure the expression levels of Cleaved-caspase-3,Bcl-2,and Bax in infarcted brain tissue.Results:Rats in the model group exhibited symptoms such as coarse fur,salivation,and poor mental state,with significantly increased mNSS scores(P<0.05),increased apoptosis in hippocampal tissue(P<0.05),elevated levels of Cleaved-caspase-3 and Bax(P<0.05),and reduced Bcl-2 expression(P<0.05).Compared to the model group,rats in the Wanshi Yutan Decoction groups showed symptom improvement(P<0.05),decreased mNSS scores(P<0.05),reduced apoptosis in hippocampal tissue(P<0.05),significantly lower levels of Cleaved-caspase-3 and Bax(P<0.05),and higher Bcl-2 expression levels(P<0.05).Conclusion:Wanshi Yutan Decoction may alleviate symptoms of ischemic stroke in rats,potentially through a mechanism related to the reduction of cell apoptosis in brain tissue.

关 键 词：缺血性脑卒中 万氏愈瘫汤 细胞凋亡 活化型半胱氨酸天冬氨酸蛋白酶-3 

分 类 号：R285.5[医药卫生—中药学]