野菊花HPLC指纹图谱及化学模式识别的研究  

Study on the HPLC Fingerprint and Chemical Pattern Recognition of Wild Chrysanthemum

作　　者：吴金富 吕涵 金阳[1] 王艳艳[1] 郭勇 李丹 黄莉莉[1] WU Jinfu;LV Han;JIN Yang;WANG Yanyan;GUO Yong;LI Dan;HUANG Lili(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Shenwei Pharmaceutical Group Co.,Ltd.,Shijiazhuang 051432,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]神威药业集团有限公司,河北石家庄051432

出　　处：《中医药学报》2025年第3期37-42,共6页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(82003974);黑龙江省博士后科研启动金(LBH-Q13161);黑龙江省自然科学基金(LH2019h106);黑龙江中医药中青年科技攻关项目(ZQG-042)。

摘　　要：目的:建立野菊花的高效液相色谱(HPLC)指纹图谱并同时结合化学模式识别,对不同产地、批次的野菊花质量进行评价研究。方法:使用Waters Symmery C18色谱柱(4.6 mm×250 mm,5μm),采用的流动相为乙腈-0.1%磷酸水溶液,进行梯度洗脱,流速选择0.6 mL·min^(-1),柱温为25℃,紫外检测波长为334 nm,进样量为10μL,结合多种化学模式识别方法对多批次野菊花进行整体质量评价。结果:15批野菊花药材指纹图谱共标定18个共有峰,相似度均>0.9,共指认蒙花苷、新绿原酸等11个共有峰。采用聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)将15批野菊花分为3大类,标记出2个主成分,筛选出3个差异质量标志物,分别为异绿原酸A、异绿原酸C、蒙花苷,可作为控制野菊花药材质量的指标性成分。结论:成功建立的野菊花HPLC指纹图谱方法具备简便、准确、良好重复性和可靠性的特点,为野菊花药材及其制剂的质量控制和临床应用提供了可靠的参考依据。Objective:To establish the High-Performance Liquid Chromatography(HPLC)fingerprint of wild chrysanthemum and evaluate the quality of wild chrysanthemum from different origins and batches using chemical pattern recognition.Methods:The study utilized a Waters Symmetry C18 column(4.6 mm×250 mm,5μm)with a mobile phase of acetonitrile-0.1%phosphoric acid aqueous solution for gradient elution.The flow rate was set at 0.6 mL·min-1,the column temperature at 25℃,and the UV detection wavelength at 334 nm,with an injection volume of 10μL.Various chemical pattern recognition methods were used for the overall quality evaluation of multiple batches of wild chrysanthemum.Results:The HPLC fingerprints of 15 batches of wild chrysanthemum identified 18 common peaks,with similarities all greater than 0.9.Eleven common peaks,including linarin and neochlorogenic acid,were identified.Using Hierarchical Cluster Analysis(HCA),Principal Component Analysis(PCA),and Orthogonal Partial Least Squares-Discriminant Analysis(OPLS-DA),the 15 batches of wild chrysanthemum were categorized into three major groups,identifying two principal components and three differential quality markers:isochlorogenic acid A,isochlorogenic acid C,and linarin,which can be used as quality control indicators for wild chrysanthemum.Conclusion:The successfully established HPLC fingerprint method for wild chrysanthemum is simple,accurate,and has good reproducibility and reliability.It provides a reliable reference for the quality control and clinical application of wild chrysanthemum medicinal materials and preparations.

关 键 词：野菊花 高效液相色谱指纹图谱 质量评价 化学模式识别 

分 类 号：R284.1[医药卫生—中药学]