基于微卫星技术的东北马鹿粪便样品的个体识别  

Microsatellite Techniques-based Analysis of Feces Samples for Individual Identification of Northeastern Red Deer

作　　者：刘艳华[1] 郑平平 霍继尧 黄玉兰 王嘉霖 李家贺 LIU Yanhua;ZHENG Pinging;HUO Jiyao;HUANG Yulan;WANG Jialin;LI Jiahe(College of Wildlife and Nature Reserves,Northeast Forestry University,Harbin Heilongjiang 150040,China)

机构地区：[1]东北林业大学野生动物与自然保护地学院,黑龙江哈尔滨150040

出　　处：《绿洲农业科学与工程》2025年第1期128-136,共9页Oasis Agriculture Science and Engineering

基　　金：世界自然基金会项目(10000766);东北林业大学校级大学生创新训练项目资助(202410225584)。

摘　　要：本研究采用QIA-GEN公司生产的粪便DNA提取试剂盒(QIAamp DNA Stool Mini Kit)进行马鹿粪便样品的提取,检测27对马鹿近缘种鹿科动物微卫星座位的多态性分布,评估其遗传多样性,并探讨其用于个体识别的可行性。结果表明:11个微卫星位点的平均杂合度在0.513-0.715之间变动,基因杂合度最小位点是T507,基因杂合度最大的位点有2个,分别是BM848和T530,平均为0.614。多态性信息含量值(PIC)在0.264-0.764之间变动,最小的为BM848,最大的是T501,平均值为0.595,其中CSSM19、T123、T156、N、BM203、OCAM、T507、T530、T501和C143平均PIC在0.5以上,为高度多态位点;BM848位点的PIC值在0.25~0.5之间,为中度多态位点。11个位点无偏随机识别概率P(ID)为1.408×10^(-3),累计个体鉴别力为99.99%。因此,这11个位点完全能够满足马鹿个体识别分析的要求。This study used the fecal DNA extraction kit(QIAamp DNA Stool Mini Kit)produced by QIA-GEN Company to extract fecal samples of red deer.The polymorphism distribution of 27 microsatellite loci of deer species closely related to red deer was detected,their genetic diversity was evaluated,and the feasibility of using them for individual identification was explored.The results showed that the average heterozygosity of 11 microsatellite loci varied between 0.513 and 0.715.The locus with the lowest gene heterozygosity was T507,and there were two loci with the highest gene heterozygosity,namely BM848 and T530,with an average of 0.614.The polymorphism information content(PIC)values varied between 0.264 and 0.764.The smallest was BM848,and the largest was T501,with an average value of 0.595.Among them,the average PIC values of CSSM19,T123,T156,N,BM203,OCAM,T507,T530,T501 and C143 were above 0.5,which were highly polymorphic loci.The PIC value of the BM848 locus was between 0.25 and 0.5,which was a moderately polymorphic locus.The unbiased random identification probability P(ID)of the 11 loci was 1.408×10^(-3),and the cumulative individual discrimination power was 99.99%.Therefore,these 11 loci can fully meet the requirements for individual identification analysis ofred deer.

关 键 词：个体识别 马鹿 粪便 微卫星 

分 类 号：Q953[生物学—动物学]