复合益生菌对食叶草全混合饲料发酵品质和体外发酵参数的影响  

Effect of compound probiotics on fermented quality and parameters in vitro of total mixed feed of protein grass

作　　者：李勇 赵庆枫[2] 王赛赛 王泽仁 张伟彬 王汝都[1] 高延玲 LI Yong;ZHAO Qingfeng;WANG Saisai;WANG Zeren;ZHANG Weibin;WANG Rudu;GAO Yanling

机构地区：[1]河南农业职业学院,河南郑州451450 [2]周口职业技术学院,河南周口466000 [3]河南奥德瑞思牧业科技有限公司,河南郑州450000 [4]周口市动植物疫病预防控制中心,河南周口466000

出　　处：《饲料研究》2025年第2期155-160,共6页Feed Research

基　　金：河南省科技攻关项目(项目编号:242102111025);河南农业职业学院家畜生态养殖科研创新团队(项目编号:ZZ2405TD03)。

摘　　要：试验旨在探究复合益生菌对食叶草全混合饲料发酵品质和体外发酵参数的影响。试验设置2组,每组5个重复,对照组不添加益生菌,复合益生菌组添加0.05%复合益生菌(枯草芽孢杆菌+酿酒酵母菌+植物乳杆菌+屎肠球菌)。两组饲料均在室温(平均气温15~25℃)下持续发酵14 d。结果显示,与对照组相比,复合益生菌组显著提高发酵饲料色泽评分、气味评分、pH值评分和总评分(P<0.05),显著提高乳酸、乙酸、丙酸、总氮、粗蛋白和可溶性碳水化合物的含量(P<0.05),显著降低pH值、丁酸、氨氮、中性洗涤纤维、酸性洗涤纤维的含量和氨氮/总氮(P<0.05)。与对照组相比,复合益生菌发酵组显著提高了瘤胃液乙酸、丙酸、戊酸及总挥发性脂肪酸含量(P<0.05),显著降低了瘤胃液pH值和氨氮含量(P<0.05)。研究表明,在食叶草全混合发酵饲料调制中,添加枯草芽孢杆菌+酿酒酵母菌+植物乳杆菌+屎肠球菌,可以提高发酵饲料感官评分和营养价值,改善饲料发酵品质和瘤胃发酵环境。The experiment aimed to investigate the effects of compound probiotics on the fermented quality and parameters in vitro of total mixed feed of protein grass.The experiment was set up with two groups,each with five replicates.The control group did not add probiotics,while the compound probiotic group added 0.05%compound probiotics(Bacillussubtilis+Saccharomycescerevisiae+Lactobacillusplantarum+Enterococcusfaecium).Both groups of feed were fermented continuously for 14 days at room temperature(average temperature 15~25℃).The results showed that compared with the control group,the compound probiotics group significantly improved the color score,odor score,pH value score,and total score of the fermented feed(P<0.05),significantly increased the concentrations of lactic acid,acetic acid,propionic acid,total nitrogen,crude protein,and soluble carbohydrates(P<0.05),and significantly decreased the pH value,butyric acid,ammonia nitrogen,neutral detergent fiber,acid detergent fiber concentrations,and ammonia nitrogen/total nitrogen(P<0.05).Compared with the control group,the compound probiotics group significantly increased the concentrations of acetic acid,propionic acid,valeric acid,and total volatile fatty acids in the rumen fluid(P<0.05),and significantly decreased the pH value and ammonia nitrogen content of the rumen fluid(P<0.05).The study indicates that adding compound probiotics such as Bacillus subtilis+Saccharomyces cerevisiae+Lactobacillus plantarum+Enterococcus faecium in the preparation of whole mixed fermented feed of protein grass can significantly improve the sensory score and nutritional value of the fermented feed,and improve the feed fermentation quality and rumen fermentation environment.

关 键 词：复合益生菌 食叶草 发酵品质 体外发酵参数 

分 类 号：S816.6[农业科学—饲料科学]