贝莱斯芽孢杆菌与化学药剂协同防治烟草棒孢霉叶斑病  

Control Effect of Bacillus velezensis Combined with Chemical Fungicide on Tobacco Leaf Spot Caused by Corynespora cassiicola

作　　者：芶剑渝 王浩强 杨相 温明霞 龙星黔 余传兴 黄纯杨 王依非 龚治乾 周红江 GOU Jianyu;WANG Haoqiang;YANG Xiang;WEN Mingxia;LONG Xingqian;YU Chuanxing;HUANG Chunyang;WANG Yifei;GONG Zhiqian;ZHOU Hongjiang(Zunyi Company of Guizhou Tobacco Company,Zunyi 563000,Guizhou,China;Anhui Agricultural University,Hefei 230036,China;Zheng'an Branch,Zunyi Company of Guizhou Tobacco Company,Zheng'an 563400,Guizhou,China)

机构地区：[1]贵州省烟草公司遵义市公司,贵州遵义563000 [2]安徽农业大学,合肥230036 [3]贵州省烟草公司遵义市公司正安分公司,贵州正安563400

出　　处：《中国烟草科学》2025年第1期56-63,69,共9页Chinese Tobacco Science

基　　金：贵州省烟草公司遵义市公司科技项目(2022XM09)。

摘　　要：为探究芽孢杆菌与化学药剂协同防治烟草棒孢霉叶斑病的可行性,采用抑菌圈法和平板计数法分析化学药剂与贝莱斯芽孢杆菌Y19的相容性,在此基础上根据Wadley法确定复配比例,探讨复配剂对烟草棒孢霉叶斑病的防效及对叶际真菌群落结构的影响。结果表明,贝莱斯芽孢杆菌Y19与38%吡唑醚菌酯·啶酰菌胺悬浮剂的生物相容性最好。Y19(1×10^(7) CFU/mL)与38%吡唑醚菌酯·啶酰菌胺(15 mg/L)复配剂体积比为2∶8时,对多主棒孢霉的抑菌效果最佳,毒力比率为1.21。室内盆栽试验结果表明,复配剂防效(57.6%)高于38%吡唑醚菌酯·啶酰菌胺和生防菌Y19单剂的防效。扩增子测序分析表明,病原菌侵染时,菌药复配剂处理能提高烟草叶际真菌群落多样性和丰富度。综上,贝莱斯芽孢杆菌Y19与38%吡唑醚菌酯·啶酰菌胺悬浮剂复配能有效防治烟草棒孢霉叶斑病,防效优于单剂。To explore the control synergism of Bacillus spp.combined with chemical fungicide against tobacco leaf spot disease caused by Corynespora cassiicola,the compatibility of chemical fungicide and Bacillus velezensis Y19 was analyzed by using inhibition zone and plate counting methods,followed by the determination of the compouding ratio with Wadley's method.The efficacy of the compounding agents and its effects on phyllospheric fungal community were investigated.Results showed that Y19 exhibited optimal biocompatibility with 38%pyrazolesterin-pyrazolamidine suspension.The mixture of Y19(1×10^(7) CFU/mL)and 38%pyrazolesterin-pyrazolamidine suspension(15 mg/L)with the volume ratio of 2∶8 exhibited a notable inhibitory effect and yielded a synergism ratio(SR)value of 1.21.Indoor pot experiment showed that the efficacy of mixed agents(57.6%)was higher than that of both 38%pyrazolesterin-pyrazolamidine suspension and Y19 individually.Amplicon sequencing analysis revealed that mixed agent treatment enhanced the diversity and abundance of tobacco pollosphere fungal community during pathogen infection.In conclusion,the combination of Bacillus subtilis Y19 and 38%pyraclostrobin-difenoconazole suspension can effectively control tobacco leaf spot disease caused by Corynespora cassiicola,with superior efficacy to the individual agents.

关 键 词：烟草 多主棒孢霉 贝莱斯芽孢杆菌 38%吡唑醚菌酯·啶酰菌胺悬浮剂 叶际微生物 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]