甲状腺细针吸取细胞学检测BRAF V600E的价值及应用研究  

Value and application of thyroid fine needle aspiration cytology in detecting BRAF V600E

作　　者：程松 杨雪英 顾亮 Cheng Song;Yang Xueying;Gu Liang(Yingtan People's Hospital,Yingtan,Jiangxi 335000,China)

机构地区：[1]江西省鹰潭市人民医院,江西鹰潭335000

出　　处：《首都食品与医药》2025年第5期90-92,共3页Capital Food Medicine

基　　金：鹰潭市指导性科技计划项目目(编号:2023-9-33260)。

摘　　要：目的 观察甲状腺细针吸取细胞学检测BRAF V600E的应用效果.方法 病例资料由鹰潭市人民医院检验科提供,收集时间为2023年4月-2023年12月,根据纳入、排除标准纳入120例行甲状腺B超引导穿刺患者为调查对象,分析所有受试者细胞学染色涂片及手术切除石蜡切片,经BRAF V600E检测后计算细胞学染色涂片的诊断效能.结果 研究纳入的120例患者均由手术切除进行诊断,其中有41例经手术切除石蜡切片行BRAF V600E检测;石蜡切片结果显示阳性32例、阴性9例,细胞学染色涂片结果显示阳性34例、阴性7例,以前者结果为金标准,经计算后可知细胞学染色涂片灵敏度、特异度、准确度分别为96.88%、66.67%、90.24%;将阳性病例CT值进行配对t检验,差异具有显著性(P<0.05).结论 甲状腺细针吸取细胞学技术效果明显,经BRAF V600E突变检测后可提高术前甲状腺疾病的检出率.Objective To observe the application effect of thyroid fine needle aspiration cytology in detecting BRAF V600E.Methods According to inclusion and exclusion criteria,120 patients who underwent B ultrasound-guided thyroid fine needle aspiration cytology at Laboratory Department of Yingtan People's Hospital from April to December 2023 were selected.All patients'stained cytological smears and paraffin sections of surgical resection specimens were analyzed.The diagnostic efficacy of stained cytological smears was evaluated.Results All patients enrolled completed diagnosis by surgical resection.Among them,41 cases experience detection of BRAF V600E using paraffin sections.Detection results of paraffin sections showed 32 positive cases and 9 negative cases.Stained smears showed that 34 positive cases and 7 negative cases.With the former as the gold standard,the sensitivity,specificity and accuracy of stained cytological smears were 96.88%,66.67%and 90.24%.Paired t-test of CT values of positive cases found that the differences were statistically significant(P<0.05).Conclusion Thyroid fine needle aspiration cytology is efficient,and BRAF V600E mutation detection can improve the preoperative detection rate of thyroid disease.

关 键 词：甲状腺细针 细胞学检测 BRAF V600E 

分 类 号：R581[医药卫生—内分泌]