天龙竭对博来霉素诱导的肺间质纤维化大鼠血管重构的影响  

Effects of Tianlongjie on vascular remodeling in rats with bleomycin-induced interstitial lung fibrosis

作　　者：袁德政 罗婷 陈冰 陆毓 刘玉凤 王子聿 刘璟 杨胜英 姚舒雅 邢海晶 付义 YUAN Dezheng;LUO Ting;CHEN Bing;LU Yu;LIU Yufeng;WANG Ziyu;LIU Jing;YANG Shengying;YAO Shuya;XING Hajing;FU Yi(Yunnan University of Chinese Medicine,Kunming 650500,China;The Third Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650500,China;The Fourth Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shengyang 110101,China;Kunming College,Kunming650214,China)

机构地区：[1]云南中医药大学,昆明650500 [2]云南中医药大学第三附属医院,昆明650500 [3]辽宁中医药大学附属第四医院,沈阳110101 [4]昆明学院,昆明650214

出　　处：《中华中医药杂志》2025年第2期636-641,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金地区科学基金项目(No.81360581);云南省中医联合重点项目(No.202101AZ070001-169);云南省高校基础研究联合专项(No.2019FH001-046);云南省教育厅科学研究基金研究生项目(No.2023Y2435)。

摘　　要：目的:通过观察天龙竭对博来霉素诱导的肺间质纤维化大鼠血管重构的影响,探讨天龙竭抗肺间质纤维化的作用机制。方法:将60只Wistar大鼠随机分为空白组,模型组,天龙竭高、中、低剂量组,吡非尼酮组,每组10只。除空白组外,其余组均采用气管内滴注博来霉素法复制肺间质纤维化大鼠模型。造模后第7天予相应药物灌胃干预。HE和Masson染色分别观察肺组织肺泡炎和肺间质纤维化的程度;免疫组织化学技术检测肺组织CD31、CD34蛋白表达,RT-PCR法检测肺组织血管生长内皮因子(VEGF)、碱性成纤维细胞生长因子(bFGF)、缺氧诱导因子-1α(HIF-1α)及Ⅰ型胶原(COL-Ⅰ)、Ⅲ型胶原(COL-Ⅲ)mRNA表达。结果:天龙竭高、中、低剂量均可抑制肺间质纤维化大鼠肺部肺泡炎和肺纤维化的发生。与模型组比较,各给药组均能显著降低肺组织中CD31、CD34蛋白表达及VEGF、bFGF、HIF-1α、COL-Ⅰ、COL-ⅢmRNA表达(P<0.01,P<0.05)。结论:天龙竭可通过抑制血管重构,降低肺纤维化模型大鼠肺组织中炎症发生,减少胶原沉积,而发挥抗肺间质纤维化的作用。Objective:To investigate the mechanism of anti-interstitial lung fibrosis by observing the effects of the cloud medicine Tianlongjie on vascular remodeling in rats with bleomycin-induced interstitial lung fibrosis.Methods:Sixty Wistar rats were randomly divided into a blank group,a model group,a group with high,medium and low doses of Tianlongjie,and a group with pirfenidone,with 10 rats in each group.Except for the blank group,all groups were used to replicate the rat model of interstitial lung fibrosis by intratracheal drip bleomycin injection.HE and Masson staining were used to observe the degree of alveolitis and interstitial fibrosis,respectively.Immunohistochemical techniques were used to detect the expression of CD31 and CD34 proteins,and RT-PCR was used to detect the mRNA expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),hypoxia-inducible factor 1α(HIF-1α)and collagen type I and type I(COL-I,COL-I).

关 键 词：天龙竭 博来霉素 肺间质纤维化 血管重构 

分 类 号：R73[医药卫生—肿瘤]