HPLC法测定经典名方温经汤包合物中桂皮醛和丹皮酚的含量  

Determination of cinnamaldehyde and paeonol in the inclusion complex of the classic prescription Wenjing decoction by HPLC

作　　者：肖星茹 李新晔 田雯菲 田野 李瑞明 XIAO Xingue;LI Xinye;TIAN Wenfie;TIAN Ye;LI Ruiming(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;State Key Laboratory of Chinese Medicine Modernization,Tianjin Key Laboratory of Component-based Chinese Medicine,Tasly Pharmaceutical Group Co.Ltd.,Tianjin 300410,China)

机构地区：[1]天津中医药大学,天津301617 [2]天士力医药集团股份有限公司,现代中药创制全国重点实验室,天津市组分中药重点实验室,天津300410

出　　处：《天津药学》2025年第2期143-146,共4页Tianjin Pharmacy

摘　　要：目的 建立高效液相色谱法(HPLC)同时测定温经汤包合物中桂皮醛和丹皮酚含量。方法 色谱柱为Welch Ultimate?Plus C18(4.6 mm×250 mm, 5μm);流动相为乙腈-水(33∶67);柱温30℃;检测波长275 nm;流速1.0 mL/min;进样量10μL。结果 两种指标成分的质量浓度分别在3.08~196.04μg/mL、1.29~100.98μg/mL线性关系良好(r>0.9999),重复性相对标准偏差(RSD)分别为0.69%和0.59%,精密度RSD分别为0.34%和0.55%,稳定性RSD分别为0.07%和0.10%,桂皮醛和丹皮酚的平均回收率分别为99.06%和102.28%,RSD分别为0.60%和1.71%,显示出良好的耐用性。结论 本研究建立的温经汤包合物中2种指标成分含量测定方法准确可靠,适用于温经汤包合物的质量评价。Objective To establish a high performance liquid chromatography(HPLC)for the simultaneous determination of cinnamaldehyde and paeonol in the inclusion complex of Wenjing decoction.Methods The chromatographic column used was Welch Ultimate Plus C18(4.6 mmx250 mm,5μm).The mobile phase consisted of acetonitrile-water(33:67).The col-umn temperature was set at 30℃,withadetectionwavelengthof275nm.Theflowratewas1.0mL/min,andtheinjectionvol-ume was 10μL.Results The mass concentrations of the two marker components showed good linearity in the ranges of 3.08~196.04μg/mL and 1.29~100.98μg/mL,respectively(r>0.9999).The relative standard deviations(RSD)for repeatability were 0.69%and 0.59%,for precision were 0.34%and 0.55%,and for stability were 0.07%and 0.10%.The average recov-ery rates for cinnamaldehyde and paeonol were 99.06%and 102.28%,respectively,with RSD values of 0.60%and 1.71%.The method demonstrated good robustness.Conclusion The HPLC method established in this study for the determination of the two marker components in the inclusion complex of Wenjing decoction is accurate and reliable,and is suitable for the quality e-valuation of the inclusion complex of Wenjing decoction.

关 键 词：高效液相色谱法 温经汤 包合物 桂皮醛 丹皮酚 

分 类 号：R927.2[医药卫生—药学]