阿旺绵羊肠道噬菌体多样性  

Diversity of intestinal phages in Awang sheep

作　　者：普布扎西 王新平 赵思越 王宇皓 斯郎江措 巴桑次仁 次仁普赤 唐鸿杰 李娜 柴立旭 Pubu Zhaxi;WANG Xinping;ZHAO Siyue;WANG Yuhao;Silang Jiangcuo;Basang Ciren;Ciren Puchi;TANG Hongjie;LI Na;CHAI Lixu(Changdu Animal Husbandry Station,Changdu 854000,China;Sichuan Agricultural University,Ya’an,611830 China;Agriculture and Animal Husbandry Science and Technology Promotion Service Center of Gongjue County,Changdu 854200,China;Changdu Science and Technology Bureau,Changdu 854000,China)

机构地区：[1]西藏昌都市畜牧总站,昌都854000 [2]四川农业大学,雅安611830 [3]西藏昌都市贡觉县农牧科技推广服务中心,昌都854200 [4]昌都市科学技术局,昌都854000

出　　处：《应用与环境生物学报》2025年第1期131-142,共12页Chinese Journal of Applied and Environmental Biology

基　　金：西藏昌都市科技计划项目(405001006)资助。

摘　　要：噬菌体在维持宿主肠道微环境中发挥着重要作用.基于宏基因组学方法全面研究阿旺绵羊肠道噬菌体.共注释到1050种噬菌体,主要来自10个门、11个科、225个属和1050个种.大多数噬菌体主要来自长尾病毒科Siphoviridae、短尾噬菌体科Podoviridae和肌尾噬菌体科Myoviridae,所占比例分别为29.9%、12.8%和9.1%.其中未分类到科的噬菌体占34.4%.225个属主要为塞昆克病毒属Cequinquevirus(2.8%)、痘苗病毒属Vaccinivirus(2.8%)、副痘病毒属Parapoxvirus(1.8%)和弗鲁纳病毒属Friunavirus(1.1%)等.在种水平上,1050个噬菌体注释到的种包括有尾噬菌体Caudovirales_phage(9.4%)、克拉斯噬菌体CrAss_phage(3.4%)和蛭弧菌噬菌体Bdellovibrio_phage(1.3%)等.此外,不同养殖方式之间,噬菌体群落结构存在显著差异(科水平:R=0.853,P<0.01;属水平:R=0.832,P<0.01;种水平:R=0.840,P<0.01).在α多样性指数方面,不同养殖方式的Chao1指数与Shannon指数存在着显著差异.本研究揭示了阿旺绵羊肠道噬菌体的群落结构特征以及养殖方式对肠道噬菌体群落结构的重要影响,为利用肠道噬菌体进行疾病防控提供了新的视角,也为保障阿旺绵羊肠道健康提供了重要的理论依据.(图17表1参24)Bacteriophages play important roles in maintaining the host intestinal microenvironment.Few studies have reported intestinal bacteriophages in sheep.In this study,we comprehensively explored intestinal bacteriophages in Awang sheep using metagenomics.We identified a total of bacteriophages,mainly from 11 families,225 genera,and 1050 species.Most bacteriophages belonged to Siphoviridae,Podoviridae,and Myoviridae,accounting for 29.9%,12.8%,and 9.1%,respectively.Among these,34.4%were bacteriophages that were not classiffed into families.These 225 genera were mainly Cequinquevirus(2.8%),Vaccinivirus(2.8%),Parapoxvirus(1.8%),and Friunavirus(1.1%).Among the 1050 phages,Caudovirales_phage(9.4%)was the dominant species,followed by CrAss_phage(3.4%)and Bdellovibrio_phage(1.3%).Significant differences were observed in the structure of the bacteriophage community among the different breeding models of Awang sheep(family:R=0.853,P<0.01;genus:R=0.832,P<0.01;species:R=0.840,P<0.01).Significant differences were also noted in the alpha and Shannon diversity indices among all the breeding models.This study revealed the community structure characteristics of intestinal phages in Awang sheep and the signiffcant impact of feeding models on the community structure of intestinal phages,providing a new perspective for the prevention and control of diseases using intestinal phages as well as an important theoretical basis for ensuring the intestinal health of Awang sheep.

关 键 词：阿旺绵羊 宏基因组学 噬菌体 肠道微生物 

分 类 号：S85[农业科学—兽医学]