CRISPR/Cas9基因编辑系统清除乙型肝炎病毒共价闭合环状DNA的研究进展  

Advances in eradicating covalently closed circular DNA of hepatitis B virus by the CRISPR/Cas9 gene editing system

作　　者：杨嘉茵 尹燕锋[2] Yang Jiayin;Yin Yanfeng(College of Life Sciences,Yunnan University,Kunming 650000,China;Experimental Center of Kunming,First People's Hospital,Kunming 650000,China)

机构地区：[1]云南大学生命科学学院,昆明650000 [2]昆明市第一人民医院实验中心,昆明650000

出　　处：《中华肝胆外科杂志》2025年第2期146-150,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：云南省科技厅重大科技专项计划(202302AA310018);春城计划高层次创新创业团队专项(2022SCP002);云南省教育厅科学研究基金(2024J0292);云南省科技厅昆明医科大学应用基础研究联合专项(202401AY070001-281)。

摘　　要：乙型肝炎病毒(HBV)感染后,很多患者会出现肝衰竭和肝癌等严重后遗症。探寻可从根本上清除HBV的方法成为治疗HBV感染的当务之急。规律成簇间隔短回文重复序列及其核酸酶9(CRISPR/Cas9)基因编辑系统是一种强大的基因组编辑工具。诸多研究证实,CRISPR/Cas9基因编辑系统能够清除细胞中HBV的共价闭合环状DNA,在治疗慢性HBV感染中具有很大的应用潜力。本文总结了CRISPR/Cas9系统在抗HBV感染中的应用现状和研究进展,同时探讨了其可能面临的问题。After hepatitis B virus(HBV)infection,numerous patients will develop serious sequelae such as liver failure and liver cancer.Finding a way to eradicate HBV has emerged as a top priority for the treatment of HBV infection.Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)gene editing system represents a potent tool for genome manipulation.Many studies have confirmed the capability of the CRISPR/Cas9 gene editing system to remove the covalently closed circular DNA of HBV in cells,which has great potential for application in the treatment of chronic HBV infection.This article summarizes the application status and research progress of CRISPR/Cas9 system in anti-HBV infection,and explores the challenges that may be encountered.

关 键 词：乙型肝炎病毒 规律成簇间隔短回文重复序列 CRISPR相关蛋白9 

分 类 号：R51[医药卫生—内科学]