微塑料和三氯生复合暴露致斑马鱼脂代谢紊乱  

The combined exposure to microplastics and triclosan disrupts lipid metabolism in zebrafish

作　　者：成冠辰 刘兴成 王慧利 钱秋慧 CHENG Guan-chen;LIU Xing-cheng;WANG Hui-li;QIAN Qiu-hui(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009

出　　处：《中国环境科学》2025年第3期1636-1646,共11页China Environmental Science

基　　金：国家自然科学基金资助项目(22305167)。

摘　　要：以斑马鱼为模式生物,旨在评估聚酰胺微塑料(PAMPs)和三氯生(TCS)复合暴露对斑马鱼幼鱼脂质代谢的影响.研究发现,PA对TCS展现出很强的吸附能力,168h时吸附量达到74%.在PA和TCS复合暴露下(6~120hpf),斑马鱼幼鱼产生明显表观畸形,例如心包水肿、游囊关闭、卵黄囊肿和脊柱弯曲等,畸形发生率为13%.此外,复合暴露还导致斑马鱼幼鱼体内活性氧的积聚,激发氧化应激反应.在肝脏区域,观察到脂质堆积的加剧,同时T-CHO和TG水平上升,伴随着脂质代谢相关基因表达的异常,从而证实PA和TCS复合暴露诱导了斑马鱼脂质代谢的紊乱.进一步的机制研究表明,PA和TCS的复合暴露导致线粒体膜电位下降和ATP合酶基因表达降低,导致能量供应受损,进而引发能量代谢紊乱.与单独的PA或TCS暴露相比,PA的存在显著增强了TCS对斑马鱼脂质代谢和能量代谢的干扰作用.In this study,zebrafish were used as a model organism to investigate the effects of combined exposure to polyamide microplastics(PA MPs)and triclosan(TCS)on lipid metabolism in larval zebrafish.The findings demonstrated that PA exhibited a strong adsorption capacity for TCS,with an adsorption rate reaching 74%at 168h.Exposure to PA and TCS from 6hpf to 120hpf resulted in pronounced morphological abnormalities in larval zebrafish,including pericardial edema,swim bladder closure,yolk sac edema,and spinal curvature,with a malformation rate of 13%.Moreover,combined exposure induced the accumulation of reactive oxygen species within the larvae,triggering oxidative stress responses.In the hepatic region,significant lipid accumulation was observed,accompanied by elevated levels of T-CHO and TG.These changes were further correlated with aberrant expression of genes involved in lipid metabolism,confirming that combined exposure to PA and TCS disrupted lipid metabolic processes in zebrafish.Mechanistic investigations revealed that combined exposure led to a reduction in mitochondrial membrane potential and decreased expression of ATP synthase genes,resulting in impaired energy supply and subsequent energy metabolism disruption.Notably,the presence of PA significantly amplified the disruptive effects of TCS on lipid and energy metabolism compared to exposure to either PA or TCS alone.

关 键 词：微塑料 三氯生 复合暴露 斑马鱼 脂质代谢紊乱 

分 类 号：X503.2[环境科学与工程—环境工程]