乳矿物盐组合物对糖皮质激素诱导骨质疏松症的改善作用  

Improvement Effect of Milk Mineral Salts Compositions on Glucocorticoid-Induced Osteoporosis

作　　者：汪达 桑浩捷 庞璟汐 黄智贤 于健 宁一冰 赵梦鸽 刘志刚[1] WANG Da;SANG Haojie;PANG Jingxi;HUANG Zhixian;YU Jian;NING Yibing;ZHAO Mengge;LIU Zhigang(College of Food Science and Engineering,Northwest Agriculture and Forestry University,Yangling 712100,China;Nutrition Research Institute,Junlebao Dairy Group Co.,LTD.,Shijiazhuang 050000,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]君乐宝科学营养研究院,河北石家庄050000

出　　处：《食品科技》2025年第1期67-74,共8页Food Science and Technology

基　　金：科技创新2030-“脑科学与类脑研究”重大项目(2022ZD0208100);陕西省重点研发计划项目(2023-YBNY-184)。

摘　　要：骨质疏松症(Osteoporosis,OP)是一种以骨量降低和骨组织微结构破坏为特征的代谢性骨病。牛初乳碱性蛋白(Colostrum basic protein,CBP)是一种分子质量为1~30 ku的活性蛋白,它以牛初乳为原料,经杀菌、脱脂、离心分离去除其余蛋白,再经微滤、超滤和冷冻干燥等工艺制成。CBP具有促进成骨细胞的分化矿化,进而缓解OP发病进程的潜能。文章旨在探究以乳矿物盐、维生素D、CBP三者联合而成的CBP组合物对糖皮质激素诱导的小鼠骨质疏松症(Glucocorticoid-induced osteoporosis,GIOP)的改善作用及其可能的作用途径。研究利用糖皮质激素药物地塞米松构建GIOP小鼠模型,探究CBP、乳矿物盐、维生素D组成的CBP组合物对GIOP的改善作用。结果表明,CBP及其组合物能够显著改善GIOP小鼠体质量(P<0.05)、骨小梁数(P<0.05)、骨小梁分离度(P<0.01),显著降低股骨骨骺区破骨细胞阳性数量(P<0.01)以及显著改善骨钙素水平(P<0.05)。以上研究表明,CBP组合物能够提高成骨细胞分化程度,平衡成骨细胞和破骨细胞,有效改善GIOP小鼠骨质疏松症状,并为富含CBP的乳制品的科学利用和产品开发提供理论依据。Osteoporosis(OP)is a metabolic bone disease characterized by reduced bone mass and microstructural destruction of bone tissue.Colostrum basic protein(CBP)is a molecular mass of about 1–30 ku active protein,it is made from bovine colostrum by sterilization,degreasing,centrifuge,and then by microfiltration,ultrafiltration and freeze-drying.CBP can promote the differentiation and mineralization of osteoblasts and alleviate the pathogenesis of OP.This article aims to explore the improving effect of CBP composition combined with milk mineral salt,vitamin D and CBP on glucocorticoid-induced osteoporosis(GIOP)in mice and its possible action pathways.The study first used the glucocorticoid drug dexamethasone to construct a GIOP mouse model to explore the improvement effect of a CBP composition composed of CBP,milk mineral salts,and vitamin D on osteoporosis.The results showed that CBP and its compositions could significantly improve the body weight(P<0.05),trabecular bone number(P<0.05),trabecular bone separation(P<0.01),significantly reduce the number of osteoclasts positive in epiphyseal region of femur(P<0.01),and improve the level of osteocalcin in GIOP mice(P<0.05).The above studies have shown that the CBP composition can increase osteoblast differentiation activity,balance osteoblasts and osteoclasts,effectively improve osteoporosis symptoms in GIOP mice,and provide a theoretical basis for the scientific utilization and product development of CBP-rich dairy products.

关 键 词：骨质疏松症 牛初乳碱性蛋白 乳矿物盐 维生素D 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]