机械损伤对马铃薯块茎伤口处的茉莉酸合成和传导的影响  

Effect of Mechanical Wounding on Jasmonic Acid Synthesis and Signaling at Potato(Solanum tuberosum)Tuber Wounds

作　　者：白露 杨瑞瑞 李芳红 王颖 王雪雪 张雪姣 韩叶 毕阳[1] BAI Lu;YANG Rui-Rui;LI Fang-Hong;WANG Ying;WANG Xue-Xue;ZHANG Xue-Jiao;HAN Ye;BI Yang(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,兰州730070

出　　处：《农业生物技术学报》2025年第3期513-522,共10页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(32360784)。

摘　　要：果蔬在采收和采后处理期间极易受到机械损伤,伤口处产生茉莉酸(jasmonic acid, JA)等多种植物激素,但JA是否参与马铃薯(Solanum tuberosum)损伤胁迫尚未见报道。本研究在前期损伤马铃薯块茎转录组分析的基础上,筛选与JA合成与传导相关的基因,分析这些基因的表达量和相关酶活性,测定伤口处JA含量。结果显示,损伤显著上调了块茎伤口处JA合成相关基因的表达,这些基因包括脂氧合酶(lipoxygenases, LOX)家族成员StLOX1.5、StLOX2、StLOX3.1和StLOX6,丙二烯氧化合酶(allene oxide synthase, AOS)家族成员StAOS1、StAOS2和StAOS3,丙二烯环化酶(allene oxide cyclase, AOC)家族成员StAOC,以及12-氧-二烯酸还原酶(12-oxo-phytodienoic acid reductase, OPR)家族成员StOPR1、StOPR2和StOPR3。损伤还显著提高了LOX和AOS活性,块茎伤口处的JA水平显著增加。此外,损伤显著上调了JA信号传导关键基因茉莉酸ZIM域(JAZ)家族成员StJAZ1、StJAZ2、StJAZ3、StJAZ4和StJAZ7的表达水平。综上,损伤会激活马铃薯块茎伤口处JA的产生和传导。本研究为马铃薯损伤胁迫响应机制的研究提供了理论基础。Fruit and vegetables are highly susceptible to mechanical wounding during harvest and postharvest handling,and the wounds induce the production of several phytohormones at the wounds,but involvement of jasmonic acid(JA)in potato(Solanum tuberosum)wound stress has not been reported.In this study,based on transcriptome analysis of pre-wounded potato tubers,the genes related to JA synthesis and signaling were screened,the expression of these genes and related enzyme activities were analyzed,the JA content at the wounds was determined.The results showed that the wound significantly upregulated the expression levels of genes related to JA synthesis at the tuber wound,including lipoxygenase(LOX)family members StLOX1.5,StLOX2,StLOX3.1 and StLOX6,propadiene oxidative synthase(AOS)family members StAOS1,StAOS2 and StAOS3,and propadiene cyclohexylase(AOC)family members StAOC and 12-oxo-dienoic acid reductase(OPR)family members StOPR1,StOPR2 and StOPR3.The wound also significantly increased LOX and AOS activities and significantly increased JA levels at the tuber wounds.In addition,the wound significantly upregulated the expression levels of key JA signaling genes,the jasmonate ZIM domain(JAZ)family members StJAZ1,StJAZ2,StJAZ3,StJAZ4 and StJAZ7.In conclusion,the wound activates JA synthesis and signaling in potato tuber wounds.This study provides a theoretical basis for the study of the response mechanism of potato wound stress.

关 键 词：马铃薯块茎 机械损伤 茉莉酸(JA) 合成 传导 

分 类 号：S184[农业科学—农业基础科学]