PpMYB113基因在桃花青苷合成和积累中的功能分析  

Functional Analysis of the PpMYB113 Gene in Anthocyanin Synthesis and Accumulation in Peach(Prunus persica)

作　　者：周淑婷 李茂福[1,3,4] 王华 孙佩[1,3,4] 康岩慧 孙向一 武军凯 张立彬 金万梅 ZHOU Shu-Ting;LI Mao-Fu;WANG Hua;SUN Pei;KANG Yan-Hui;SUN Xiang-Yi;WU Jun-Kai;ZHANG Li-Bin;JIN Wan-Mei(Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China;College of Horticultural Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066600;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture and Rural Affairs,Beijing 100093,China;Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China)

机构地区：[1]北京市农林科学院林业果树研究所,北京100093 [2]河北科技师范学院园艺科技学院,秦皇岛066600 [3]农业农村部华北地区园艺作物生物学与种质创制重点实验室,北京100093 [4]北京市落叶果树工程技术研究中心,北京100093

出　　处：《农业生物技术学报》2025年第3期536-546,共11页Journal of Agricultural Biotechnology

基　　金：北京市农林科学院科技创新平台建设项目(PT2023-07);北京市农林科学院科技创新能力专项(KJCX20230602);河北省现代农业产业技术体系创新团队建设(HBCT2024160203)。

摘　　要：MYB转录因子在桃(Prunus persica)果实花青苷积累过程中起着重要作用。深入研究MYB转录因子有助于理解桃次生代谢网络调控机制,为遗传改良和培育富含花青苷的新品种提供理论依据。为了探究PpMYB113在桃花和果实花青苷积累过程中的作用,本研究以红肉桃为材料,通过克隆获得PpMYB113基因(GenBank No. XM_007216468.2),对其进行生物信息学及表达模式分析,并通过在烟草(Nicotiana benthamiana)叶片和桃果肉瞬时转化对其功能进行研究。结果显示,PpMYB113基因开放阅读框为720 bp,编码239个氨基酸。进化树分析结果表明,PpMYB113蛋白与拟南芥(Arabidopsis thaliana) S6亚家族的MYBs转录因子亲缘关系最近。利用半定量RT-PCR及RT-qPCR对PpMYB113在桃花瓣、枝条、叶片、树皮、果皮和果肉中的表达进行分析,结果显示,PpMYB113在花瓣、果皮和果肉中高表达,而在枝条、叶片和树皮中不表达。利用瞬时转化体系在烟草和白色果肉桃中过表达PpMYB113,结果显示,过表达的烟草叶片和白色果肉出现红色表型,且花青苷积累增加。过表达烟草叶片和白色果肉区域,PpMYB113表达量显著高于对照组,且显著上调花色素苷合成酶(anthocyanidin synthase, ANS)和UDP-葡萄糖:类黄酮3-O-葡萄糖基转移酶(UDP glucose:flavonoid-3-O-glucosyltransferase, UFGT)基因的表达。以上结果表明,PpMYB113在桃花青苷积累中起正向促进作用。本研究为进一步揭示MYB转录因子在红肉桃花青苷积累的分子调控机制提供帮助。MYB transcription factors play a crucial role in the accumulation of anthocyanins in peach(Prunus persica)fruit.A deeper understanding of MYB transcription factors is beneficial for elucidating the molecular regulatory mechanism of secondary metabolic networks in peach,providing a theoretical basis for genetic improvement,and developing new varieties rich in anthocyanins.In this study,in order to investigate the role of PpMYB113 in the accumulation of anthocyanins in peach flowers and fruits,the PpMYB113 gene(GenBank No.XM_007216468.2)was cloned using red flesh peach as the experimental material.The bioinformatics and expression pattern analysis was performed,and the function of PpMYB113 gene was studied through transient transformation in tobacco(Nicotiana benthamiana)and peach flesh.The results showed that the open reading frame of PpMYB113 gene was 720 bp,encoding 239 amino acids.The phylogenetic tree analysis indicated that the PpMYB113 protein sequence had the closest phylogenetic relationship with the MYB transcription factors of the S6 subfamily in Arabidopsis thaliana.The expression of PpMYB113 gene was analyzed in petals,branches,leaves,bark,fruit skin,and fruit flesh of peach by using semi-quantitative RT-PCR and RT-qPCR.The results indicated that PpMYB113 gene was highly expressed in petals,fruit skin,and fruit flesh,while it was not expressed in branches,leaves,and bark.At the same time,PpMYB113 was overexpressed in tobacco leaves and white fruit flesh using the transient transformation system.The overexpressed tobacco leaves and white fruit flesh showed red color phenotype and the anthocyanin contants were significant increase.In the overexpressed tobacco leaves and white fruit flesh areas,the expression of PpMYB113 was significantly higher than that of the control,and the expression of ANS(anthocyanidin synthase)and UFGT(UDP glucose:flavonoid-3-O-glucosyltransferase)genes were significantly up-regulated.Based on these findings,it was concluded that PpMYB113 plays a positive promoting role in the acc

关 键 词：桃 MYB转录因子 表达分析 花青苷 

分 类 号：S662.1[农业科学—果树学]