薰衣草萜类合成相关基因LiMYC1的表达分析及功能验证  

Expression Analysis and Functional Verification of the LiMYC1 Related to Lavender(Lavandula×intermedia)Terpenoid Synthesis

作　　者：薛志航 买尔孜亚古丽•阿不来克木 王爱凡 廖燕 严中建 苏秀娟[1,2] 郭丹丽[3] XUE Zhi-Hang;Maierziyaguli•ABULAIKEMU;WANG Ai-Fan;LIAO Yan;YAN Zhong-Jian;SU Xiu-Juan;GUO Dan-Li(College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;Lavender Research Institute/Xinjiang Key Laboratory of Crop Biological Breeding,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Agriculture Sciences,the Fourth Agriculture Production Division of Xinjiang Production and Construction Crops,Cocodala 835219,China)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业大学薰衣草研究所/新疆作物生物育种重点实验室,乌鲁木齐830052 [3]新疆生产建设兵团第四师农科所,可克达拉835219

出　　处：《农业生物技术学报》2025年第3期570-580,共11页Journal of Agricultural Biotechnology

基　　金：新疆维吾尔自治区“天山英才”青年拔尖人才项目(2023TSYCCX0017);国家自然科学基金地区科学基金(31960452)。

摘　　要：薰衣草(Lavandula angustifolia)作为世界重要的香料经济作物,其花穗中萃取的精油蕴含了丰富的芳香化合物。这些化合物主要依赖于植物体内质体中的甲基赤藓糖醇-4-磷酸(methylerythritol 4-phosphate, MEP)途径进行代谢合成。在MEP途径中,1-脱氧-D-木酮糖-5-磷酸合酶(1-deoxy-D-xylulose 5-phosphate synthase, DXS)和1-脱氧-D-木酮糖-5-磷酸还原异构酶(1-deoxy-D-xylulose 5-phosphate reductoisomerase, DXR)作为前2步关键限速酶,对于整个代谢过程的顺利进行具有决定性的影响。为了验证MYC1在挥发性萜类化合物合成方面的功能,本研究从杂薰衣草(Lavandula×intermedia)中克隆LiMYC1 (myelocytomatosis 1)基因,通过构建pCAMBIA3301和pTRV2植物表达载体,利用根癌农杆菌(Agrobacterium tumefaciens)介导的遗传转化方法获得过表达和基因沉默植株。对转基因植株中LiMYC1、LiDXS和LiDXR基因的表达量进行检测,并对叶片中挥发性萜类化合物进行定性定量分析。结果显示,在LiMYC1基因过表达株系中,LiDXS和LiDXR基因的表达量显著上升,同时芳樟醇(linalool)和石竹烯(caryophyllene)的含量也出现不同程度的增加。相反,在LiMYC1基因沉默株系中,LiDXS和LiDXR基因的表达量明显降低,石竹烯的含量随之减少,但芳樟醇的含量却出现异常升高。以上结果表明,LiMYC1基因对萜类化合物合成的MEP途径起正调控作用,影响关键酶LiDXS和LiDXR的表达,且对石竹烯和芳樟醇的生物合成具有一定的调控作用。本研究对于解析薰衣草香气形成的分子机制,以及优化薰衣草的经济价值具有重要的指导意义。Lavender(Lavandula angustifolia)is an important spice cash crop in the world.The essential oil extracted from the inflorescence is rich in aromatic compounds.These compounds mainly rely on the methylerythritol-4-phosphate(MEP)pathway in plant plastids for metabolic synthesis.In the MEP pathway,1-deoxy-D-xylose-5-phosphate synthase(DXS)and 1-deoxy-D-xylulose 5-phosphate reductoisomerase(DXR),as the first 2 key rate-limiting enzymes,play a decisive role in the smooth progress of the whole metabolic process.The LiMYC1(myelocytomatosis 1)gene cloned from Lavandula×intermedia was targeted,and its function in the synthesis of volatile terpenoids was verified.In this study,The pCAMBIA3301 and pTRV2 plant expression vectors were constructed,and overexpression and gene-silencing plants were obtained using Agrobacterium-mediated genetic transformation methods.The expression of LiMYC1,LiDXS and LiDXR genes in transgenic plants were detected,and volatile terpenoids in leaves were qualitatively and quantitatively analyzed.The results showed that in the LiMYC1 gene overexpression line,the expression of LiDXS and LiDXR increased significantly,and the contents of linalool and caryophyllene also increased to varying degrees.On the contrary,in the LiMYC1 gene silenced strain,the expression of LiDXS and LiDXR were significantly reduced,and the content of caryophyllene was reduced,but the content of linalool was abnormally increased.The above results indicated that the LiMYC1 gene positively regulated the MEP pathway of terpenoid synthesis,affected the expression of key enzymes LiDXS and LiDXR,and had a certain regulatory effect on the biosynthesis of caryophyllene and linalool.This study has important guiding significance for analyzing the molecular mechanism of lavender aroma formation and optimizing the economic value of lavender.

关 键 词：薰衣草 萜类化合物 转录因子 功能验证 

分 类 号：S573.9[农业科学—作物学]