木麻黄海藻糖-6-磷酸合酶家族鉴定及表达分析  

Identification and Expression Analysis of Trehalose-6-Phosphate Synthase Family in Casuarina equisetifolia

作　　者：张桂华 岳春雷[1] 李贺鹏[1] 张晓勉[1] 俞振明 ZHANG Gui-Hua;YUE Chun-Lei;LI He-Peng;ZHANG Xiao-Mian;YU Zhen-Ming(Zhejiang Academy of Forestry,Hangzhou 310023,China;School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 311402,China)

机构地区：[1]浙江省林业科学研究院,杭州310023 [2]浙江中医药大学药学院,杭州311402

出　　处：《农业生物技术学报》2025年第3期581-593,共13页Journal of Agricultural Biotechnology

基　　金：浙江省省院合作林业科技项目(2022SY06);国家自然科学基金(32401543);浙江省省属科研院所扶持专项(2024F1068-2);浙江省基础公益研究计划项目(LY23H280003)。

摘　　要：海藻糖-6-磷酸合酶(trehalose-6-phosphate synthase, TPS),是海藻糖生物合成的关键限速酶,在植物非生物胁迫应答过程中起重要作用。为探究TPS在木麻黄(Casuarina equisetifolia)生长发育及逆境应答中的功能,本研究分析了木麻黄CeTPS家族成员的理化特征、染色体定位、系统发育树、保守基序、基因结构、顺式调控元件及共线性关系,并分析其在木麻黄不同组织、及干旱、盐胁迫、脱落酸(abscisic acid, ABA)处理下的表达水平。结果显示,7个木麻黄CeTPS基因随机锚定在4条染色体上,编码氨基酸长度为826~998 aa,均为亲水蛋白。进化发育树显示木麻黄CeTPS分为2个亚族,与双子叶植物亲缘关系较近,存在基因复制事件。CeTPS基因启动子上大量分布激素响应、生长发育及胁迫应答相关的顺式元件。CeTPS基因具有组织特异性表达,并受干旱、盐胁迫及ABA处理呈现差异化表达,特别是CeTPS4在木麻黄胁迫应答过程中起重要作用。研究结果为深入研究木麻黄CeTPS基因在非生物胁迫中的功能提供理论依据。Trehalose-6-phosphate synthase(TPS)is the crucial rate-limiting enzyme for the trehalose biosynthesis,and plays a significant role in the stress responsiveness.To investigate the function of TPS regarding growth and development,stress response in Casuarina equisetifolia,physicochemical features,chromosomal localization,phylogenetic tree,conserved motifs,gene structure,cis-regulatory elements and collinearity were analyzed in this study.Their expression patterns in different tissues,and exposed to drought,salt stress and abscisic acid(ABA)treatment were determined.Results indicated that 7 CeTPS genes were randomly distributed in 4 chromosomes,encoding 826~998 amino acids that were hydrophilic.The CeTPS proteins were divided into 2 subfamilies through phylogenetic analysis,showing relatively close to dicotyledonous plants,and harbored gene duplications.The promoter regions of CeTPS genes were rich in cis-acting elements involved in hormonal responsiveness,growth and development,and stress responsiveness.CeTPS genes showed tissue-specific expression,and were differentially expressed under drought,salt stress,and ABA treatment.Especially,CeTPS4 might play an essential role in stress responsiveness.The results lay a foundation for in-depth study of the CeTPS genes associated with abiotic stress responsiveness.

关 键 词：木麻黄 海藻糖-6-磷酸合酶(TPS) 表达分析 胁迫 

分 类 号：S184[农业科学—农业基础科学]