湖羊TMEM18基因的组织表达及其多态性与饲料转化率的关联分析  

Tissue Expression of TMEM18 Gene and Correlation Analysis Between Its Polymorphism and Feed Conversion Ratio in Hu Sheep(Ovis aries)

作　　者：刘晓强 李成海 王维民 高飞 田慧彬 王立忠 赵源 马宗武 黄志强 蔡有鑫 张小雪[1] LIU Xiao-Qiang;LI Cheng-Hai;WANG Wei-Min;GAO Fei;TIAN Hui-Bin;WANG Li-Zhong;ZHAO Yuan;MA Zong-Wu;HUANG Zhi-Qiang;CAI You-Xin;ZHANG Xiao-Xue(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Jinchuan Group Co.,Ltd.,Jinchang Jujia Ecological Agriculture Co.,Ltd.,Jinchang 737100,China;College of Grassland Agricultural Science and Technology/State Key Laboratory of Grassland Agroecosystems,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]金川集团股份有限公司金昌居佳生态农业有限公司,金昌737100 [3]兰州大学草地农业科技学院/草种创新与草地农业生态系统全国重点实验室,兰州730000

出　　处：《农业生物技术学报》2025年第3期608-615,共8页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2022YFD1302000);甘肃省科技重大专项(22ZD6NC069);金川集团股份有限公司2023年重大科研项目(金科综2023-13);节粮低脂湖羊的选育和研究(JKQGJJ23Z202301)。

摘　　要：跨膜蛋白18(transmembrane protein 18,TMEM18)是影响动物生长发育的重要候选基因,编码具有重要生物学功能的跨膜蛋白。为了探究TMEM18基因在绵羊(Ovis aries)组织中的表达模式和饲料转化率的关系,寻找可用于育种的分子遗传标记。本研究以845只系谱清晰、饲料效率性状记录完整的湖羊群体作为研究对象,通过qRT-PCR技术检测了TMEM18基因在湖羊10个组织中的表达水平,采用Sanger测序技术扫描了该基因的SNPs,并与饲料转化率进行了关联分析。结果显示,TMEM18基因在心脏中的表达量显著高于其他组织(P<0.05),在十二指肠、瘤胃、肺脏和肝脏等组织中具有较高的表达量。在绵羊TMEM18基因的第3内含子中检测到TMEM18 g.517832 T>C突变位点。多重比较结果显示,该突变位点与湖羊FCR100~120、FCR140~160、FCR80~120、FCR140~180、FCR100~160、FCR80~160、FCR100~180和FCR80~180显著关联(P<0.05)。其中,CC基因型个体的FCR140~160、FCR100~160、FCR80~160、FCR100~180和FCR80~180显著高于TT基因型和TC基因型的个体(P<0.05)。以上结果表明,TMEM18g.517832 T>C位点可以作为候选分子标记用于提高绵羊的饲料转化率。本研究为湖羊节粮型品种选育提供基因材料。Transmembrane protein 18(TMEM18)is an important candidate gene for animal growth and development,encoding a transmembrane protein with important biological functions.In order to explore the expression pattern of TMEM18 gene in sheep(Ovis aries)tissues,the correlation between it's polymorphism and feed conversion ratio(FCR),and molecular genetic markers for breeding were searched.Based on a population of 845 Hu sheep with clear pedigrees and complete phenotypic records,this research utilized qRT-PCR technology to detect the expression levels of the TMEM18 gene in 10 tissues of Hu sheep and employed Sanger sequencing technology to scan for SNPs in this gene,which were then analyzed for association with feed efficiency traits.The results showed that the relative expression of the TMEM18 gene was significantly higher in the heart than in other tissues(P<0.05),followed by high relative expression in the duodenum,rumen,lungs,and liver.A synonymous mutation polymorphism site,g.517832 T>C,was detected in the third intron of the sheep TMEM18 gene.The results of multiple comparisons showed that the mutation locus was significantly associated with FCR100~120,FCR140~160,FCR80~120,FCR140~180,FCR100~160,FCR80~160,FCR100~180 and FCR80~180(P<0.05),among them,FCR140~160,FCR100~160,FCR80~160,FCR100~180 and FCR80~180 with CC genotype were significantly higher than those of TT genotype and TC genotype(P<0.05).The results of this study suggested that TMEM18 g.517832 T>C site could be used as a candidate molecular marker to improve feed efficiency in sheep.This study provides reliable genetic materials for the breeding of grain-saving varieties.

关 键 词：跨膜蛋白18(TMEM18)基因 饲料转化率 湖羊 SNPS 

分 类 号：S813.3[农业科学—畜牧学]