云南樟基质添加对牛樟芝生长特性的影响  

Effects of Cinnamomum glanduliferum Matrix Addition on the Growth Characteristics of Antrodia cinnamomea

作　　者：宝元朵 黄兴连 杨志娟 武自强 张颖 郑元 Bao Yuanduo;Huang Xinglian;Yang Zhijuan;Wu Ziqiang;Zhang Ying;Zheng Yuan(Yunnan Provincial Key Laboratory of Forest Disaster Early Warning and Control,College of Forestry,Southwest Forestry University,Kunming Yunnan 650233,China;Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Kunming Yunnan 650223,China;College of Biological Science and Food Engineering,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学林学院,西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650233 [2]中国科学院西双版纳热带植物园热带植物资源与可持续利用重点实验室,云南昆明650223 [3]西南林业大学生物与食品工程学院,云南昆明650233

出　　处：《西南林业大学学报(自然科学)》2025年第1期26-33,共8页Journal of Southwest Forestry University:Natural Sciences

基　　金：国家自然科学基金项目(32160736)资助;云南省农业基础研究联合专项重点项目(202101BD070001-020)资助;云南省基础研究计划面上项目(202101AT070044)资助;“兴滇英才支持计划”青年人才专项项目(XDYC-QNRC-2022-0245)资助。

摘　　要：为探讨云南樟基质添加对牛樟芝生长特性的影响,在马铃薯琼脂培养基上添加云南樟10种浓度(0.125~64 g/L)的嫩枝、嫩叶及其混合物,恒温培养30 d,分析其菌落直径、菌丝密度、菌落颜色、菌落长势、菌丝生长速度、生长指数、生物量、SOD活性、TT含量的差异。结果表明:在添加云南樟嫩枝条件下,当添加浓度为1~8 g/L时,牛樟芝的生长特性和生物量较优;添加浓度分别为2~4 g/L和2 g/L时,牛樟芝的SOD活性和TT含量显著优于CK。在添加云南樟嫩叶条件下,当添加浓度分别为1~4、1~16 g/L时,其生长特性和生物量较优;添加浓度为0.5~2 g/L时,其SOD活性和TT含量显著优于CK。在添加云南樟枝叶混合物条件下,当添加浓度分别为2~16、4~16、16~32、8~32 g/L时,其生长特性、生物量、SOD活性和TT含量均保持在较优水平。可见,添加云南樟4 g/L嫩叶基质,对牛樟芝生长指标和生物量的促进效果最优,其菌落直径达83.40 mm,菌丝生长速度达2.78 mm/d,生物量达0.23 g;添加云南樟2 g/L嫩枝基质,显著提高了牛樟芝的SOD活性;添加云南樟16 g/L枝叶混合基质,则显著促进了牛樟芝的总三萜含量。To explore the effect of Cinnamomum glanduliferum substrate addition on the growth and physiological and biochemical characteristics of Antrodia cinnamomea.Potato agar medium(PDA)was supple-mented with 10 concentrations(0.125–64 g/L)of C.glanduliferum branches,leaves and their mixtures and cul-tured at constant temperature for 30 days,and the differences in colony diameter,mycelial density,colony color,colony growth potential,mycelial growth rate,growth index,biomass,superoxide dismutase(SOD)activity,and total triterpene(TT)content were analyzed.The findings indicated that under the condition of adding C.glanduliferum branches in PDA medium,the growth characteristics and biomass of A.cinnamomea were higher when the addition concentration was 1–8 g/L,and the SOD activity and TT content of A.cinnamomea were signi-ficantly higher when the addition concentration was 2–4 g/L and 2 g/L,respectively;under the condition of adding young leaves of C.glanduliferum to PDA medium,its growth characteristics and biomass were higher when the concentration was 1–4 g/L and 1–16 g/L,respectively.When the concentration was 0.5–2 g/L,its SOD activity and TT content were significantly higher than those of the control;the growth characteristics,biomass,SOD activ-ity and TT content were kept at a high level when the concentration of C.glanduliferum branches and leaves mix-ture addition was 2–16 g/L,4-–16 g/L,16–32 g/L and 8–32 g/L,respectively.It follows that adding 4 g/L of C.glanduliferum young leaf as the substrate has the best promoting effect on the growth indicators and biomass of A.cinnamomea,with a colony diameter of 83.40 mm,a mycelial growth rate of 2.78 mm/d,and a biomass of 0.23 g;adding 2 g/L of C.glanduliferum branches matrix significantly increased the SOD activity of A.cinnamomea;the addition of 16 g/L mixed substrate of C.glanduliferum branches and leaves significantly promoted the total triter-pene content of A.cinnamomea.

关 键 词：牛樟芝 云南樟 固体培养 SOD 总三萜 

分 类 号：S481[农业科学—农药学]