基于单细胞转录组的水稻稀有气孔细胞类型鉴定  

Identification of Rare Stomatal Cell Types in the Single-cell Transcriptome of Rice

作　　者：刘开燕 孙桂玲 郭振华[1,2] Liu Kaiyan;Sun Guiling;Guo Zhenhua(Germplasm Bank of Wild Species in Southwest China,Kunming Institute of Botany,Chinese Academic of Sciences,Kunming Yunnan 650201,China;University of Chinese Academic of Sciences,Beijing 100049,China;CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Kunming Yunnan 650223,China)

机构地区：[1]中国科学院昆明植物研究所,中国西南野生生物种质资源库,云南昆明650201 [2]中国科学院大学,北京100049 [3]中国科学院西双版纳热带植物园,热带植物资源可持续利用重点实验室,云南昆明650223

出　　处：《西南林业大学学报(自然科学)》2025年第1期44-54,共11页Journal of Southwest Forestry University:Natural Sciences

基　　金：国家自然科学基金面上项目(32270716)资助。

摘　　要：为探讨禾本科植物气孔发育和运动的调控机制,在初次聚类的基础上,利用获得的所有表皮细胞进行亚聚类分析;同时结合更多标记基因,在亚聚类Resolution值为2.6时成功地从水稻叶片单细胞转录组数据中获得了独立且稳定的保卫细胞和副卫细胞的聚类,分别包括了12个保卫细胞和17个副卫细胞。GO富集分析揭示了“基于维管的运动”“信号转导”和“对刺激的响应”等气孔后期发育的生物学特征;基因调控网络分析揭示了OsDOF11、OsMAPK2、OsNAC75、OsABCG4、OsERF52和OsSWEET2b等基因及其潜在的相互作用网络。研究结果为植物稀有细胞类型的鉴定提供了方法参考,同时也为其他禾本科植物气孔细胞的研究提供了新的标记基因。To explore the regulatory mechanism of development and movement of grass stomata,on the basis of the primary clustering,the study successfully obtained independent and stable clusters of guard cells and subsi-diary cells from single-cell transcriptome data in rice leaves by sub-clustering analysis of all the obtained epiderm-al cells when the resolution value of sub-clustering was set at 2.6,combined with more marker genes.They con-sist of 12 guard cells and 17 subsidiary cells,respectively.GO enrichment analysis revealed the biological pro-cesses of late stomatal development such as"microtubule-based movement","signal transduction"and"response to stimulus".Gene regulatory network analysis revealed genes such as OsDOF11,OsMAPK2,OsNAC75,OsAB-CG4,OsERF52 and OsSWEET2b and their potential interaction networks.The results of this study provide a methodological reference for the identification of rare cell types in plants,and also provides new marker genes for the study of stomatal cells in other grasses.

关 键 词：稀有细胞类型 气孔 水稻 单细胞转录组 

分 类 号：S511[农业科学—作物学]