金环胡蜂和黄脚胡蜂表皮化学成分差异分析  

Difference Analysis of the Chemical Composition of the Cuticular of Vespa mandarinia and V.velutina

作　　者：向清东 蒋学建[2] 赵鹏飞 王维招 欧正文 杨振德[1] Xiang Qingdong;Jiang Xuejian;Zhao Pengfei;Wang Weizhao;Ou Zhengwen;Yang Zhende(College of Forestry,Guangxi University,Nanning Guangxi 530004,China;Guangxi Forest Pests Natural Enemies Breeding Research Center of Engineering Technology,Guangxi Academy of Forestry Sciences,Nanning Guangxi 530002,China;Nanning Huxin Biotechnology Co.,Ltd.,Nanning Guangxi 530002,China)

机构地区：[1]广西大学林学院,广西南宁530004 [2]广西壮族自治区林业科学研究院,广西林业有害生物天敌繁育工程技术研究中心,广西南宁530002 [3]南宁市互信生物科技有限公司,广西南宁530002

出　　处：《西南林业大学学报(自然科学)》2025年第1期166-173,共8页Journal of Southwest Forestry University:Natural Sciences

基　　金：广西重点研发计划项目(桂科AB21220004)资助;胡蜂蜜蜂混养模式研究项目(桂林科研[2022]第16号)资助;胡蜂蜂毒产品的开发及利用研究项目(桂林科研[2022ZC]第1号)资助。

摘　　要：通过诱捕法收集金环胡蜂和黄脚胡蜂2种活体昆虫,利用气相色谱-质谱联用技术,分析2种胡蜂表皮化合物的主要成分。结果表明:从2种胡蜂表皮中共鉴定出32种化合物,以烃类、羧酸类和醇类为主,占测定化合物种类数量的70.97%;2种胡蜂表皮化合物的种类和含量均有差异,金环胡蜂表皮中相对含量最多的5种化合物为13-甲基二十七烷(32.31%)、2-甲基二十四烷(23.53%)、三十二烷(14.61%)、(E)-9-十八烯酸(12.80%)、胆固醇(5.70%),黄脚胡蜂表皮中相对含量最多的5种化合物为二十烷(22.45%)、亚磷酸三(2,4-二叔丁基苯基)酯(19.13%)、三十二烷(17.55%)、(E)-9-十八烯酸(15.66%)、13-甲基二十七烷(5.52%);2种胡蜂表皮相对含量最高的5种化合物中,2-甲基二十四烷只存在于金环胡蜂中,三十二烷和(E)-9-十八烯酸在2种胡蜂表皮中相对含量接近,二十烷、13-甲基二十七烷、亚磷酸三(2,4-二叔丁基苯基)酯在2种胡蜂表皮中相对含量差异较大。对2种胡蜂表皮化合物的研究有助于进一步揭示其生活习性和行为特征,可为更好开展虫害防治和绿色防控提供参考。The Vespa mandarinia and V.velutina were collected using trapping methods,and the main com-ponents of the cuticular compounds of the 2 wasps were analyzed with gas chromatography-mass spectrometry.The results showed that a total of 32 compounds were identified from the cuticular of the 2 wasps,dominated by hydrocarbons,carboxylic acids and alcohols,which accounted for 70.97%of the determined compound species.The types and contents of cuticular compounds differed between the 2 wasp species.The 5 most abundant com-pounds in V.mandarinia were 13-Methylheptacosane(32.31%),2-Methyltetracosane(23.53%),Dotriacontane(14.61%),(E)-9-Octadecenoic acid(12.80%),and Cholesterol(5.70%),while the 5 most abundant compounds in V.velutina were Eicosane(22.45%),Tris(2,4-ditert-butylphenyl)phosphite(19.13%),Dotriacontane(17.55%),(E)-9-Octadecenoic acid(15.66%),and 13-Methylheptacosane(5.52%).Of the 5 compounds with the highest relative amounts in the 2 wasps,2-Methyltetracosane was present only in the V.Mandarinia.The relative contents of tri-decane and(E)-9-Octadecenoic acid were relatively close in the cuticular of the 2 wasps,while the relative con-tents of Eicosane,13-Methyloctadecanes,Tris(2,4-ditert-butylphenyl)phosphite had large variations in the cuticu-lar of the 2 wasps.The study of the cuticular compounds of the 2 wasp species can help to further reveal their habits and behavioural characteristics,with a view to providing a reference for better pest control and green pre-vention and control.

关 键 词：金环胡蜂 黄脚胡蜂 表皮 化合物 主要成分 GC-MS 

分 类 号：S718[农业科学—林学]