白星花金龟幼虫粪中除氨菌的筛选与鉴定  

Screening and Identification of Ammonia-removing Bacteria in the Feces of Protaetia brevitarsis Larvae

作　　者：刘业超 王庆雷[2,3] 刘雪英 赵鹏武 晁开瑞[1] 张居宇 姚湘 陈萌 张燕如 Liu Yechao;Wang Qinglei;Liu Xueying;Zhao Pengwu;Chao Kairui;Zhang Juyu;Yao Xiang;Chen Meng;Zhang Yanru(College of Forestry,Inner Mongolia Agricultural University,Hohhot Inner Mongolia 010019,China;Cangzhou Academy of Agricultural and Forestry Sciences,Cangzhou Hebei 061001,China;Key Laboratory of Soil Entomology in Hebei Province,Cangzhou Hebei 061001,China)

机构地区：[1]内蒙古农业大学林学院,内蒙古呼和浩特010019 [2]沧州市农林科学院,河北沧州061001 [3]河北省土壤昆虫学重点实验室,河北沧州061001

出　　处：《西南林业大学学报(自然科学)》2025年第1期174-180,共7页Journal of Southwest Forestry University:Natural Sciences

基　　金：内蒙古自治区自然科学基金项目(2020BS03014)资助;国家自然科学基金项目(32160372)资助;内蒙古自治区林学“双一流”建设项目(DC2100001171)资助;内蒙古自治区林业科技推广示范项目资助。

摘　　要：为了从白星花金龟幼虫虫粪中分离筛选具有除氨效果的微生物,为有机废弃物转化利用提供微生物资源。采用富集驯化、初筛、复筛相结合的方式从白星花金龟幼虫虫粪中筛选出2株除氨菌种N1、N2,结合形态、生理生化、16S r DNA分子鉴定菌株类别,对菌株的生理性状和生长曲线进行测定;并通过硼酸吸收凯氏定氮法测定单菌株和复配菌株在25 d内对NH_(3)释放的影响。结果表明:N1和N2均表现出了明显的除氨能力,复配菌株的除氨效果高于单菌株。经鉴定N1为小麦苍白杆菌,N2为苍白杆菌属。N1和N2生长曲线各具特点。两菌株在30~37℃pH=5~9时生长情况最好。从白星花金龟幼虫虫粪中分离的2株菌株能有效降低NH3释放量,2株菌株复配的除氨效果高于单菌株,可作为有机废弃物在发酵腐熟期间有效的除氨微生物。To isolate and screen microorganisms with ammonia removal effect from the feces of the larvae of Protaetia brevitarsis,and to provide microbial resources for the conversion and utilization of organic waste,2 strains of ammonia-removing bacteria N1 and N2 were screened from the feces of the larvae of P.brevitarsis by means of enrichment and domestication,preliminary screening and re-screening.The strains were identified by morphological,physiological and biochemical,and 16SrDNA molecular identification.The physiological charac-teristics and growth curve of the strain were determined.The effects of single strain and compound strain on NH_(3) release within 25 days were determined by boric acid absorption Kjeldahl method.The findings indicated that both N1 and N2 showed obvious ammonia removal ability,and the ammonia removal effect of the compound strain was higher than that of the single strain.N1 was identified as Ochrobactrum tritici,and N2 was identified as Ochrobactrum sp.The growth curves of N1 and N2 had their own characteristics.The 2 strains grew best at 30℃–37℃ pH=5–9.The 2 strains isolated from the larvae of P.brevitarsis can effectively reduce the release of NH3,and the ammonia removal effect of the 2 strains is higher than that of the single strain,which can be used as an effective ammonia removal microorganism during the fermentation of organic waste.

关 键 词：白星花金龟 幼虫虫粪 微生物 鉴定 除氨菌 

分 类 号：Q93.331[生物学—微生物学]