不同光质对樟叶越桔组培苗生长及其6'-O-咖啡酰熊果苷含量的影响  

Effects of Different Light Quality on the Growth of Tissue Culture Seedlings of Vaccinium dualianum and the Production of 6'-O-caffeoylarbutinfeoylarbutin

作　　者：萧卫泓 李楚然 孙蕊 王卫华 刘云[2] 杨晓琴[1] 朱国磊 解思达 赵平[1,2] Xiao Weihong;Li Churan;Sun Rui;Wang Weihua;Liu Yun;Yang Xiaoqin;Zhu Guolei;Xie Sida;Zhao Ping(Key Laboratory of National Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming Yunnan 650233,China;Key Laboratory of Ministry of Education for Forest Resources Conserva-tion and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming Yunnan 650233,China;College of Agronomy and Life Science,Zhaotong University,Zhaotong Yunnan 657000,China)

机构地区：[1]西南林业大学西南地区林业生物质资源高效利用国家林业和草原局重点实验室,云南昆明650233 [2]西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明650233 [3]昭通学院农学与生命科学学院,云南昭通657000

出　　处：《西南林业大学学报(自然科学)》2025年第1期190-197,共8页Journal of Southwest Forestry University:Natural Sciences

基　　金：国家自然科学基金项目(32060107,32060327)资助。

摘　　要：通过对樟叶越桔组培苗的生长生理指标及其中6'-O-咖啡酰熊果苷(CA)含量的测定,分析蓝色、红色、不同红蓝色配比(1∶1、1∶2和2∶1)及白色(对照)LED光对其生长和主要次生代谢物的影响。结果表明:单色红光处理可促进组培苗苗高的增长,蓝光处理可提高组培苗丙二醛(MDA)含量及过氧化物酶(POD)活性;红蓝光复合配比有利于提高叶绿素含量和增加对光胁迫抗逆性,在红蓝光比为1∶1时组培苗具有较高的超氧化物歧化酶(SOD)活性。当红光比例增加时,组培苗的可溶性糖及可溶性蛋白含量呈现先增加后减少的趋势;当蓝光比例增加时,组培苗的MDA含量和POD活性均为上升趋势;当红蓝光比为1∶2时,组培苗中CA的含量最高[(21.02±0.56) mg/g],为对照组的1.5倍,而在其他光质处理下其含量均下降。研究结果可为进一步阐明樟叶越桔CA的生产调控机制提供参考。In this study,the effect of red,blue,different ratios of red and blue(1∶1,1∶2,and 2∶1),and white(control group)LED light on the growth and primary secondary metabolites of tissue culture seedlings in Vaccinium dualianum was investigated by measuring the physiological parameters and the content of 6'−O−caf-feoylarbutin(CA).The results showed that the growth of seedling height was promoted under the treatment of monochromatic red light,and MDA content and POD activity of seedlings were enhanced under the treatment of monochromatic blue light.The chlorophyll content and resistance to seedlings'light stress were increased under the combination of red and blue light.Of these,the tissue culture seedlings had a higher SOD activity when the ra-tio of red and blue light was 1∶1.The soluble sugar and soluble protein contents of tissue culture seedlings in-creased first and then decreased with the increase of red light proportion,while the MDA content and POD activ-ity enhanced with the proportion of blue light increased.The highest content of CA in tissue culture seedlings was(21.02±0.56)mg/g when the ratio of red to blue was 1∶2,which was 1.5 fold higher compared to the control group,while its content decreased under other light-quality treatments.The results could a provide the reference for the regulation mechanism of CA production in V.dunalianum.

关 键 词：光质 樟叶越桔 组培苗 生理指标 红光 蓝光 6'-O-咖啡酰熊果苷 

分 类 号：TQ041[化学工程]