基于内标法的低质量浓度葡萄糖溶液拉曼测定研究  

Raman Determination of Low Mass Concentration Glucose Solutions Based on Internal Standard Method

作　　者：杜康 花扬扬 魏帅飞 王婷婷[1,2] 曲冠男 赵猛[1,2] 蔡红星[1,2] Du Kang;Hua Yangyang;Wei Shuaifei;Wang Tingting;Qu Guannan;Zhao Meng;Cai Hongxing(School of Physics,Changchun University of Science and Technology,Changchun 130022,Jilin,China;Key Laboratory of Jilin Province for Spectral Detection Science and Technology,Changchun University of Science and Technology,Changchun 130022,Jilin,China)

机构地区：[1]长春理工大学物理学院,吉林长春130022 [2]长春理工大学吉林省光谱探测科学与技术重点实验室,吉林长春130022

出　　处：《光学学报(网络版)》2024年第5期4-10,共7页Acta Optica Sinica(Online)

基　　金：吉林省自然科学基金(YDZJ202201ZYTS510);吉林省教育厅科学研究项目(JJKH20230795KJ)

摘　　要：为提升葡萄糖溶液的检测性能,首先对葡萄糖溶液中体积分数为99%的吡喃葡萄糖进行仿真计算,获得理论拉曼光谱;其次,使用拉曼光谱系统获取不同质量浓度葡萄糖溶液的实验拉曼光谱,理论计算结果显示,两种吡喃葡萄糖在1125 cm^(-1)处均有特征峰,与实验结果一致;再次,选择水的1640 cm^(-1)特征峰作为内标峰进行归一化;最后,采用特征峰相对强度和特征峰相对面积两种定量分析方法进行线性分析,线性相关系数分别为0.992和0.985,最低检测限为48.1 mg/dL和52.3 mg/dL,最低检测质量浓度均为12.5 mg/dL。结果表明,相对强度法简单方便,线性拟合度更高,更适用于低质量浓度葡萄糖溶液的精确定量分析。To improve the detection performance of glucose solutions,we first simulate and calculate the theoretical Raman spectrum of 99%(volume fraction)glucopyranose in the glucose solution.Next,we obtain experimental Raman spectra of glucose solutions at different mass concentrations using a Raman spectroscopy system.The theoretical calculations show that both types of glucopyranose exhibit characteristic peaks at 1125 cm^(−1),which is consistent with the experimental results.We then select the 1640 cm^(−1) characteristic peak of water as the internal standard peak for normalization.Finally,two quantitative analysis methods,the characteristic peak relative intensity method and the characteristic peak relative area method,are used for linear analysis.The linear correlation coefficient are 0.992 and 0.985,respectively.The lowest detection limits are 48.1 mg/dL and 52.3 mg/dL,and the lowest detection mass concentrations are all 12.5 mg/dL.The results indicate that the relative intensity method is simple and convenient,and provides a higher linear fitting,making it more suitable for accurate quantitative analysis of low mass concentration glucose solutions.

关 键 词：光谱学 拉曼光谱 葡萄糖 内标法 

分 类 号：O433.4[机械工程—光学工程]