不同剂量布鲁氏菌S2疫苗免疫奶山羊后血清抗体消长规律研究  

Study on the fluctuation of serum antibodies of dairy goats immunized with different doses of Brucella S2 vaccine

作　　者：张雅婷 陈兰 陈昕迪 王腾宇 曹天宇 于宇 孟宪然 王文龙[1] Zhang Yating;Chen Lan;Chen Xindi;Wang Tengyu;Cao Tianyu;Yu Yu;Meng Xianran;Wang Wenlong(College of Veterinary Medicine,Inner Mongolia Agricultural University,Inner Mongolia Hohhot 010011;Ordos Animal Disease Prevention and Control Center,Inner Mongolia Ordos 017000;Dongsheng District Agricultural and Livestock Product Quality and Safety Workstation,Inner Mongolia Ordos 017000)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010011 [2]鄂尔多斯市动物疫病预防控制中心,内蒙古鄂尔多斯017000 [3]东胜区农畜产品质量安全工作站,内蒙古鄂尔多斯017000

出　　处：《现代畜牧兽医》2025年第1期10-14,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：内蒙古自治区科技重大专项(2021ZD0023)。

摘　　要：试验旨在探索口服不同剂量布鲁氏菌S2疫苗进行免疫后奶山羊血清抗体消长规律,试将26只5月龄奶山羊随机分为两组,每组13只羊。一倍剂量组、二倍剂量组口服10^(10)、2.0×10^(10) CFU布鲁氏菌S2疫苗,并分别于免疫前和免疫后第3、5、7、15、21、30、60、90、120、150、180、210、240天采血,采用虎红平板凝集试验(RBPT)、试管凝集试验(SAT)及间接酶联免疫吸附试验(iELISA)监测奶山羊血清抗体阳性率变化情况。结果显示:试验羊口服一倍剂量S2疫苗免疫后第7天开始产生抗体;第21天时RBPT、SAT、iELISA测得抗体阳性率达到最高,分别为84.6%、84.6%、92.3%;免疫后第90天时大部分试验羊抗体转阴,RBPT、SAT和iELISA测得抗体阳性率均为23.1%;免疫第210天后3种检测方法测定抗体结果均为阴性。试验羊口服二倍剂量S2疫苗免疫后第5天开始产生抗体;免疫后第15天,SAT、iELISA测得抗体阳性率达到最高(92.3%),免疫后第21天,RBPT测得抗体阳性率达到最高(92.3%);免疫后第30天时抗体阳性率呈下降趋势;免疫后第90天时,大部分试验羊抗体转阴,RBPT、SAT和iELISA测得抗体阳性率分别为15.4%、15.4%、23.1%;免疫后第180天时,3种检测方法测定抗体结果均为阴性。研究表明,布鲁氏菌S2疫苗免疫后能可使奶山羊较快产生抗体且抗体能够维持半年,口服一倍剂量和二倍剂量得到的免疫效果无明显差别。This study was conducted to investigate the change of serum antibodies in dairy goats immunized with different doses of Brucella S2 vaccine.26 dairy goats aged 5 months were randomly divided into two groups with 13 sheep in each group.The one-dose and two-dose groups received 10^(10) and 2.0×10^(10) CFU Brucella S2 vaccine orally,and blood samples were collected before and after immunization on days 3,5,7,15,21,30,60,90,120,150,180,210,240,respectively.Tiger Red plate agglutination test(RBPT),test tube agglutination test(SAT)and indirect enzyme-linked immunosorbent assay(iELISA)were used to monitor the changes of serum antibody positive rate of dairy goats.The results showed that the antibody began to be produced on the 7th day after oral immunization with double dose of S2 vaccine.The positive rates of RBPT,SAT and iELISA were 84.6%,84.6%and 92.3%on the 21th day,respectively.On the 90th day after immunization,most of the antibodies turned negative,and the positive rates of antibodies measured by RBPT,SAT and iELISA were 23.1%.After 210 days of immunization,the results of the three detection methods were negative.Experimental goats produced antibodies on 5th day after oral immunization with two-dose of Brucella S2 vaccine.On the 15th day after immunization,the antibody positive rate measured by SAT and iELISA reached the highest(92.3%),and on the 21st day after immunization,the antibody positive rate measured by RBPT reached the highest(92.3%).The positive rate of antibody decreased on the 30th day after immunization.On the 90th day after immunization,most of the antibodies turned negative,and the positive rates of antibodies measured by RBPT,SAT and iELISA were 15.4%,15.4%,and 23.1%,respectively.At 180 days after immunization,the antibody results of the three methods were negative.Studies have shown that the Brucella S2 vaccine can make dairy goats produce antibodies quickly after immunization and antibodies can be maintained for half a year,and there is no significant difference in the immune effect between on

关 键 词：布鲁氏菌病 奶山羊 布鲁氏菌S2疫苗 抗体消长规律 

分 类 号：S855[农业科学—临床兽医学]