壳聚糖与γ-聚谷氨酸对三种海洋微藻的絮凝收获作用研究  

Study on Harvesting Three Species of Marine Microalgae via Flocculation Using Chitosan andγ-Polyglutamic Acid

作　　者：潘凯琳 郑怡鸿 赖俊翔[3,4] 李智巧 姜发军[3,4] PAN Kailin;ZHENG Yihong;LAI Junxiang;LI Zhiqiao;JIANG Fajun(School of Resources,Environment and Materials,Guangxi University,Nanning 530004,China;College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518060,China;Guangxi Key Laboratory of Offshore Marine Environmental Science(Guangxi Mangrove Research Center of Guangxi Academy of Marine Sciences),Guangxi Academy of Sciences,Nanning 530007,China;Fangchenggang Branch of Guangxi Academy of Sciences(Beibu Gulf Marine Industry Research Institute),Fangchenggang 538000,China)

机构地区：[1]广西大学资源环境与材料学院,广西南宁530004 [2]深圳大学生命与海洋科学学院,广东深圳518060 [3]广西科学院,广西海洋科学院(广西红树林研究中心),广西近海海洋环境科学重点实验室,广西南宁530007 [4]广西科学院防城港分院(北部湾海洋产业研究院),广西防城港538000

出　　处：《湖南生态科学学报》2025年第1期74-80,共7页Journal of Hunan Ecological Science

基　　金：广西创新驱动发展专项(桂科AA18242047)。

摘　　要：【目的】本研究旨在评估壳聚糖(CTS)与γ-聚谷氨酸(PGA)作为絮凝剂对海水小球藻、杜氏盐藻、紫球藻三种海洋微藻的絮凝收获效果,并探究不同絮凝剂用量及投加次序对絮凝效率的影响,以期为开发环保高效的微藻收获技术提供参考。【方法】本研究采用CTS和PGA作为絮凝剂,对海水小球藻、杜氏盐藻、紫球藻三种海洋微藻进行絮凝实验。实验包括CTS单独使用以及联合PGA的复合使用,通过调整絮凝剂用量及投加次序,观察并记录其对絮凝效果的影响。【结果】对于海水小球藻和杜氏盐藻,CTS与PGA的联合使用显著增强了絮凝效果。其中,先投加CTS再投加PGA的复合方式表现最佳,海水小球藻的絮凝率高达96.62%,盐藻的絮凝率则达到87.54%。对于盐藻,在最佳投加次序下,CTS的最佳作用浓度为150 mg/L(絮凝率为78.45%),PGA的最佳作用浓度为75 mg/L(絮凝率为68.92%)。对于紫球藻,CTS与PGA的联合使用及CTS单独使用均能有效提高其絮凝效果。其中,CTS单独作用时效果最佳,最佳作用浓度为150 mg/L(絮凝率为99.18%)。【结论】本研究表明,采用先投加CTS再投加PGA的联合使用策略,能有效提高三种海洋微藻的絮凝效果。这一发现为开发环保高效的微藻收获技术提供了有价值的参考,并为未来的研究提供了方向。【Objective】The aim of this study was to evaluate the effect of chitosan(CTS)andγ-polyglutamic acid(PGA)as flocculants on the harvesting of three kinds of marine microalgae,namely,Chlorella sp.,Dunaliella salina,and Porphyridium cruentum,and to investigate the effects of different flocculant dosage and accession sequences on the flocculation efficiency with the aim of providing a reference to the development of environmentally friendly and efficient microalgae harvesting technology.【Method】In this study,CTS and PGA were used as flocculants to flocculate three kinds of marine microalgae,namely Chlorella sp.,D.salina,and P.cruentum.The experiments included the use of CTS alone and the combined use of PGA and CTS,and the effects on the flocculation effect were explored by adjusting the dosage of flocculants and the order of dosing.【Result】For Chlorella sp.and D.salina,the combined use of CTS and PGA significantly enhanced the flocculation effect.Among them,the combination of CTS followed by PGA showed the best performance in the experiment of different sequences of flocculants,with the flocculation rate of Chlorella sp.reaching 96.62%and that of D.salina reaching 87.54%.For D.salina,under the optimal dosing sequence,the optimal concentration of CTS was 150 mg/L(flocculation rate of 78.45%),and the optimal concentration of PGA was 75 mg/L(flocculation rate of 68.92%).In the case of P.cruentum,both the combined use of CTS and PGA and the use of CTS alone could effectively improve the flocculation effect.Among them,the best effect was achieved when CTS was used alone,and the optimum concentration of CTS was 150 mg/L(flocculation rate of 99.18%).【Conclusion】This study showed that the combined use strategy of CTS followed by PGA can effectively improve the flocculation effect of Chlorella sp.,D.salina;as to P.cruentum,the best harvesting can achieve in the case of CTS used alone.This finding provided a valuable reference for the development of environmentally friendly and efficient microalgae harvesting t

关 键 词：壳聚糖 Γ-聚谷氨酸 絮凝 双絮凝剂 微藻收获 

分 类 号：Q949.2[生物学—植物学] X71[环境科学与工程—环境工程]